Wioland M, Mehrishi J N
Scand J Immunol. 1979;10(5):453-63. doi: 10.1111/j.1365-3083.1979.tb01375.x.
The distribution of the anodic electrophoretic mobilities (EPM) of human peripheral blood lymphocytes was determined for lymphocytes isolated from umbilical cord blood and from blood of individuals 6 months to 93 years of age. The distribution was bimodal in infants up to 2 years of age and suggested a small percentage of cells with a mobility of 0.95 micrometer s-1 V-1 cm. this value was chosen to discriminate between low-mobility cells (LMCs) and high-mobility cells (HMCs). The relative percentage of LMCs increased from birth to 2 years and two types of LMCs could be distinguished. The distribution was unimodal and asymmetric in children and adults and nearly Gaussian in aged people. Substantial differences between the distributions of the lymphocyte EPMs were seen on comparison of the histograms for individuals of similar ages. The analysis of the distribution of the lymphocyte EPMs on cell suspensions enriched in, or depleted of T or B cells confirmed the mobility of most T cells to be higher than the mobility of most B cells, whatever the age of the individual. The distribution of lymphocyte EPMs determined in the same adult over a 6 year period showed minor variations.
测定了从脐带血以及6个月至93岁个体的血液中分离出的人外周血淋巴细胞的阳极电泳迁移率（EPM）分布。在2岁以下的婴儿中，该分布呈双峰状，表明有一小部分细胞的迁移率为0.95微米·秒⁻¹·伏特⁻¹·厘米。该值被用于区分低迁移率细胞（LMC）和高迁移率细胞（HMC）。LMC的相对百分比从出生到2岁有所增加，并且可以区分出两种类型的LMC。在儿童和成人中，分布是单峰且不对称的，而在老年人中接近高斯分布。比较相似年龄个体的直方图时，可以看到淋巴细胞EPM分布之间存在显著差异。对富含或缺乏T细胞或B细胞的细胞悬液中淋巴细胞EPM分布的分析证实，无论个体年龄如何，大多数T细胞的迁移率都高于大多数B细胞。在同一名成年人6年期间测定的淋巴细胞EPM分布显示出微小变化。
