Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P. 19046 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil.
Departamento de Química, Universidade Federal do Paraná, Cx.P. 19032 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil.
Biochim Biophys Acta Proteins Proteom. 2020 Feb;1868(2):140320. doi: 10.1016/j.bbapap.2019.140320. Epub 2019 Nov 20.
In recent years, metagenomic strategies have been widely used to isolate and identify new enzymes from uncultivable components of microbial communities. Among these enzymes, various lipases have been obtained from metagenomic libraries from different environments and characterized. Although many of these lipases have characteristics that could make them interesting for application in biocatalysis, relatively little work has been done to evaluate their potential to catalyze industrially important reactions. In the present article, we highlight the latest research on lipases obtained through metagenomic tools, focusing on studies of activity and stability and investigations of application in biocatalysis. We also discuss the challenges of metagenomic approaches for the bioprospecting of new lipases.
近年来，宏基因组策略已被广泛用于从微生物群落中不可培养的成分中分离和鉴定新的酶。在这些酶中，已经从不同环境的宏基因组文库中获得了各种脂肪酶，并对其进行了表征。尽管许多这些脂肪酶具有使其在生物催化中具有应用价值的特性，但在评估其在工业上重要反应中的催化潜力方面，相对较少的工作。在本文中，我们重点介绍了通过宏基因组工具获得的脂肪酶的最新研究，重点研究了其活性和稳定性，并探讨了其在生物催化中的应用。我们还讨论了宏基因组方法在新脂肪酶生物勘探方面的挑战。
