Rodríguez-Campos Daniel, Rodríguez-Melcón Cristina, Alonso-Calleja Carlos, Capita Rosa
Department of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, Spain.
Institute of Food Science and Technology, University of León, E-24071 León, Spain.
Pathogens. 2019 Nov 20;8(4):250. doi: 10.3390/pathogens8040250.
Some strains of can persist in food-processing environments, increasing the likelihood of the contamination of foodstuffs. To identify traits that contribute to bacterial persistence, a selection of persistent and sporadic isolates from a poultry-processing facility was investigated for biofilm-forming ability (crystal violet assay). The susceptibility of sessile cells to treatments (five minutes) with sodium hypochlorite having 10% active chlorine (SHY: 10,000 ppm, 25,000 ppm, and 50,000 ppm) and benzalkonium chloride (BZK: 2500 ppm, 10,000 ppm, and 25,000 ppm) was also studied. All isolates exhibited biofilm formation on polystyrene. Persistent strains showed larger ( < 0.001) biofilm formation (OD = 0.301 ± 0.097) than sporadic strains (OD = 0.188 ± 0.082). A greater susceptibility to disinfectants was observed for biofilms of persistent strains than for those of sporadic strains. The application of SHY reduced biofilms only for persistent strains. BZK increased OD in persistent strains (2500 ppm) and in sporadic strains (all concentrations). These results indicate that the use of BZK at the concentrations tested could represent a public health risk. Findings in this work suggest a link between persistence and biofilm formation, but do not support a relationship between persistence and the resistance of sessile cells to disinfectants.
某些菌株可在食品加工环境中持续存在，增加了食品污染的可能性。为了确定有助于细菌持续存在的特性，对从一家家禽加工厂挑选出的持续性和偶发性分离株进行了生物膜形成能力（结晶紫测定法）研究。还研究了固着细胞对含10%活性氯的次氯酸钠（SHY：10000 ppm、25000 ppm和50000 ppm）和苯扎氯铵（BZK：2500 ppm、10000 ppm和25000 ppm）处理（五分钟）的敏感性。所有分离株在聚苯乙烯上均表现出生物膜形成。持续性菌株的生物膜形成量（OD = 0.301±0.097）比偶发性菌株（OD = 0.188±0.082）更大（<0.001）。观察到持续性菌株的生物膜比偶发性菌株的生物膜对消毒剂更敏感。SHY的应用仅能减少持续性菌株的生物膜。BZK可增加持续性菌株（2500 ppm）和偶发性菌株（所有浓度）的OD值。这些结果表明，在所测试的浓度下使用BZK可能存在公共卫生风险。这项工作的研究结果表明持续性与生物膜形成之间存在联系，但不支持持续性与固着细胞对消毒剂的抗性之间存在关联。
