Leclercq Alexandre, Moura Alexandra, Vales Guillaume, Tessaud-Rita Nathalie, Aguilhon Christine, Lecuit Marc
3Inserm U1117, Paris, France.
1Institut Pasteur, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
Int J Syst Evol Microbiol. 2019 Jan;69(1):74-81. doi: 10.1099/ijsem.0.003097. Epub 2018 Nov 20.
During a screening of Listeria species in food samples in Thailand, a Listeria-like bacterium was recovered from fried chicken and could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene and on 243 Listeria core genes placed the novel taxon within the Listeria aquatica, Listeria floridensis, Listeria fleishmannii and Listeria costaricensis clade (Listeria sensu lato), with highest similarity to L. floridensis (98.9 %) and L. costaricensis (98.8 %). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI<86 %), the pairwise amino acid identity (AAI>64 %) and on the percentage of conserved proteins (POCP>77 %) with currently known Listeria species confirmed that the strain constituted a new taxon within the genus Listeria. At the phenotypical level, it differs from other Listeria species by the production of acid from d-tagatose and inositol. The name Listeria thailandensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2015/00305 (=CIP 111635=DSM 107638).
在泰国对食品样本中的李斯特菌属进行筛查期间，从炸鸡中分离出一种类似李斯特菌的细菌，无法将其归为任何已知物种。基于16S rRNA基因和243个李斯特菌核心基因的系统发育分析将该新分类单元置于水生李斯特菌、佛罗里达李斯特菌、弗莱施曼李斯特菌和哥斯达黎加李斯特菌分支（广义李斯特菌）内，与佛罗里达李斯特菌（98.9%）和哥斯达黎加李斯特菌（98.8%）相似度最高。基于平均核苷酸比对一致性（ANI<86%）、成对氨基酸一致性（AAI>64%）以及与当前已知李斯特菌物种的保守蛋白百分比（POCP>77%）进行的全基因组序列分析证实，该菌株构成了李斯特菌属内的一个新分类单元。在表型水平上，它与其他李斯特菌物种的不同之处在于能利用d-塔格糖和肌醇产酸。提议将该新物种命名为泰国李斯特菌（Listeria thailandensis sp. nov.），其模式菌株为CLIP 2015/00305（=CIP 111635=DSM 107638）。
