Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medial University, Luzhou 646000, Sichuan, PR China; Department of Pharmacy, The First Naval Force Hospital of Southern Theatre Command, Zhanjiang 524005, Guangdong, PR China.
State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200437, PR China; Shanghai Professional and Technical Service Center for Biological Material Drug-ability Evaluation, Shanghai 200437, PR China.
Int J Biol Macromol. 2020 Jun 1;152:1186-1193. doi: 10.1016/j.ijbiomac.2019.10.210. Epub 2019 Nov 20.
It is widely accepted that regulating microbiome could improve human health. We previously observed apple polysaccharide (AP) reversed high-fat-induced microbial dysbiosis, but the mechanism remains to be elucidated. In this study, the function of AP in vitro was evaluated in Bifidobacterium longum (B. longum) and Lactobacillus rhamnosus (L. rhamnosus). The effects of AP on the composition of fecal bacteria of normal SD rats were investigated by qPCR, TA cloning and 16S sequencing. 0.125-2% AP showed no significant effect on the growth of B. longum and L. rhamnosus. DNA concentration of fecal bacteria cultured with 1% AP was significantly higher than that of control group. qPCR revealed that the number of Bifidobacterium and Lactobacillus in fecal flora incubated by 1% AP was significantly higher than that of control group. Three strains of escherichia coli (E. coli) in fecal bacteria were screened out and analyzed. AP can be utilized by one E. coli and the metabolic products of AP could enhance the proliferation of B. longum. These data suggest that AP could promote the growth of B. longum indirectly, and provide another basis to understand the health care function of apple.
人们普遍认为，调节微生物组可以改善人类健康。我们之前观察到苹果多糖（AP）可以逆转高脂肪引起的微生物失调，但机制仍有待阐明。在这项研究中，我们评估了 AP 在长双歧杆菌（B. longum）和鼠李糖乳杆菌（L. rhamnosus）中的体外功能。通过 qPCR、TA 克隆和 16S 测序研究了 AP 对正常 SD 大鼠粪便细菌组成的影响。0.125-2%的 AP 对 B. longum 和 L. rhamnosus 的生长没有显著影响。用 1%AP 培养的粪便细菌的 DNA 浓度明显高于对照组。qPCR 显示，用 1%AP 孵育的粪便菌群中双歧杆菌和乳酸杆菌的数量明显高于对照组。筛选并分析了粪便细菌中的三株大肠杆菌（E. coli）。AP 可被一种大肠杆菌利用，AP 的代谢产物可促进 B. longum 的增殖。这些数据表明，AP 可以间接促进 B. longum 的生长，为理解苹果的保健功能提供了另一个依据。
