Institute of Tissue Transplantation and Immunology, Department of Immunobiology, Jinan University, Guangzhou 510632, China; Key Laboratory of Functional Protein Research of Guangdong, Higher Education Institutes, Jinan University, Guangzhou, China.
Institute of Tissue Transplantation and Immunology, Department of Immunobiology, Jinan University, Guangzhou 510632, China.
Toxicol In Vitro. 2020 Mar;63:104720. doi: 10.1016/j.tiv.2019.104720. Epub 2019 Nov 22.
Photoinitiators have widely been applied to industry, and now increasingly to clinical therapy. A novel high-performance photoinitiation system based on π-conjugated dithienophosphole derivatives (DTPs), including Ph-DTP and TPA-DTP, has recently been developed with high extinction coefficients and amazing polymerization initiating abilities at a relatively low dose in both the near-UV and visible light ranges, and TPA-DTP has better absorption characteristics than Ph-TPA. The purpose of this study was to evaluate cytotoxicity of DTPs and cytocompatibility of their initiated polymers by a MTT assay and calcein AM/propidium iodide staining. The cytotoxicity of Ph-DTP or TPA-DTP exposed to non-irradiation or irradiation is lower than bis (2,4,6-trimethylbenzoyl)-phenylphosphine oxide (BAPO) applied widely to industry. No obvious cytotoxicity was observed in the TPA-DTP-treated primary cells and various cell lines, while the activated Ph-DTP inhibited the cell viability and resulted in the massive cellular apoptosis and necrosis. Compared to camphorquinone (CQ) applied widely to clinical dental prosthetic restoration, the cytocompatibility of DTPs-photopolymerized BisGMA/TEGDMA polymers was inferior to CQ although their extracts exhibited low toxicity, indicating that TPA-DTP but not Ph-DTP is more suitable for industrial application. These results provide a novel insight into underlying potential application of DTPs to industrial deep polymerization or clinical tissue engineering rehabilitation.
光引发剂已广泛应用于工业领域，现在越来越多地应用于临床治疗。最近开发了一种基于π共轭二噻吩并磷杂环戊烯（DTP）衍生物的新型高性能光引发体系，包括 Ph-DTP 和 TPA-DTP，具有较高的消光系数和在近紫外和可见光范围内相对低剂量下的惊人聚合引发能力，而 TPA-DTP 比 Ph-TPA 具有更好的吸收特性。本研究旨在通过 MTT 测定法和 calcein AM/碘化丙啶染色法评估 DTP 的细胞毒性及其引发聚合物的细胞相容性。未辐照或辐照下的 Ph-DTP 或 TPA-DTP 的细胞毒性低于广泛应用于工业的双（2,4,6-三甲基苯甲酰基）-苯基氧化膦（BAPO）。在未处理的原代细胞和各种细胞系中，未观察到 TPA-DTP 处理的明显细胞毒性，而活化的 Ph-DTP 抑制了细胞活力并导致大量细胞凋亡和坏死。与广泛应用于临床牙科修复体的樟脑醌（CQ）相比，尽管 DTPs-光聚合 BisGMA/TEGDMA 聚合物的提取物表现出低毒性，但它们的细胞相容性不如 CQ，表明 TPA-DTP 比 Ph-DTP 更适合工业应用。这些结果为 DTP 在工业深度聚合或临床组织工程修复中的潜在应用提供了新的见解。
