University of Belgrade, School of Dental Medicine, DentalNet Research Group, Rankeova 4, Belgrade, Serbia; University of Belgrade, Institute of Nuclear Sciences "Vinča", P.O. Box 522, Belgrade, Serbia.
University of Belgrade, Institute of Nuclear Sciences "Vinča", P.O. Box 522, Belgrade, Serbia.
Dent Mater. 2017 Apr;33(4):454-466. doi: 10.1016/j.dental.2017.02.002. Epub 2017 Feb 28.
To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects.
Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ, DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ, BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle.
Individual substances showed decreasing cytotoxic effects in the following order: BisGMA>TPO>FIT>CQ>DMAEMA>TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO>BisGMA>FIT>CQ>TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO.
Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater biocompatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.
分别比较新型氨酯基单体 FIT-852 和单酰基膦氧化物光引发剂（Lucirin TPO）与传统双酚 A-缩水甘油基甲基丙烯酸酯（BisGMA）和三乙二醇二甲基丙烯酸酯（TEGDMA）单体以及樟脑醌（CQ）/胺光引发剂系统的细胞毒性和遗传毒性。此外，我们还定量分析了树脂基混合物中各物质的组合效应对组合效应性质的影响。
采用 MTT 和彗星试验检测 BisGMA、FIT、TEGDMA、CQ、DMAEMA 和 TPO 及其在四种临床相关混合物（FIT/TPO、FIT/CQ、BisGMA/TPO、BisGMA/CQ）中的联合毒性对人胎肺成纤维细胞 MRC-5 的细胞毒性和遗传毒性。我们从测量的浓度-效应关系评估单体和光引发剂对整体毒性的组合效应。基于从质量作用定律推导而来的中值效应方程计算组合指数（CI）。
各物质的细胞毒性依次降低：BisGMA>TPO>FIT>CQ>DMAEMA>TEGDMA。实验混合物的细胞毒性依次降低：BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO。FIT 基混合物中各成分之间表现出拮抗细胞毒性作用，而 BisGMA 基混合物则表现出协同作用，ED TPO 放大了混合物中的拮抗和协同细胞毒性作用。纯物质的遗传毒性依次为：TPO>BisGMA>FIT>CQ>TEGDMA。我们未检测到 DMAEMA 的遗传毒性潜力。混合物的遗传毒性浓度排序为：BisGMA/TPO>BisGMA/CQ>FIT/CQ>FIT/TPO。
FIT 的细胞毒性和遗传毒性均低于 BisGMA，表明其生物相容性更高。相反，光引发剂 TPO 的细胞毒性和遗传毒性均明显高于 CQ 和 DMAEMA。CI 值表明，FIT 基混合物的成分表现出拮抗细胞毒性作用，而 BisGMA 基混合物的成分则表现出协同作用。
