Department of Toxicology, School of Public Health, Guangxi Medical University, No. 22, Shuangyong Road, Nanning 530021, Guangxi Province, China.
Department of Radiology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
Life Sci. 2020 Jan 1;240:117091. doi: 10.1016/j.lfs.2019.117091. Epub 2019 Nov 21.
Mounting evidences indicated that elevated iron levels in the substantia nigra (SN) have been concerned as the underlying mechanisms of neurodegenerative diseases, including Parkinson's disease (PD). The present study used the 1-Methyl-4-phenyl-1, 2, 3, 6 -tetrahydropyridine (MPTP)-treated cynomolgus monkeys for PD to evaluate the usability of SWI for assessing iron deposition in the cerebral nuclei of PD. The results showed that susceptibility-weighted imaging (SWI) phase values of the ipsilateral (MPTP-lesion side) SN of MPTP-treated monkeys were lower than those in the contralateral SN of MPTP-treated monkeys and the same side of Control monkeys, suggesting that iron deposition were elevated in the affected side SN of MPTP-treated monkeys. Whereas MPTP has not effects on the SWI phase values in other detected brain regions of monkeys, including red nucleus (RN), putamen (PUT) and caudate nucleus (CA). Furthermore, ICP-MS results showed that MPTP increased the iron levels in MPTP injection side, but no in the ipsilateral striatum. Additionally, MPTP treatment did not affect the calcium and manganese levels in the detected brain regions of monkeys. However, Pearson correlation analysis results indicated that there were not relationship between SWI phase values in MPTP-lesion side of SN with the behavioral score, tyrosine hydroxylase (TH)-positive cells number and iron levels in the MPTP-lesion side of midbrain. Taken together, the results confirm the involvement of SN iron accumulations in the MPTP-treated monkey models for PD, and indirectly verify the usability of SWI for the measurement of iron deposition in the cerebral nuclei of PD.
越来越多的证据表明，纹状体（SN）中铁水平升高与神经退行性疾病（包括帕金森病（PD））的潜在机制有关。本研究使用 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）处理的食蟹猴 PD 模型来评估 SWI 用于评估 PD 患者脑核中铁沉积的可用性。结果表明，MPTP 处理的猴单侧（MPTP 损伤侧）SN 的磁敏感加权成像（SWI）相位值低于 MPTP 处理的猴对侧 SN 和对照猴的同测 SN，提示 MPTP 处理的猴受影响侧 SN 中的铁沉积增加。然而，MPTP 对猴其他检测到的脑区（包括红核（RN）、壳核（PUT）和尾状核（CA））的 SWI 相位值没有影响。此外，ICP-MS 结果表明，MPTP 增加了 MPTP 注射侧的铁水平，但对同侧纹状体没有影响。此外，MPTP 处理不影响猴检测脑区的钙和锰水平。然而，Pearson 相关分析结果表明，SN 中 MPTP 损伤侧的 SWI 相位值与行为评分、酪氨酸羟化酶（TH）阳性细胞数量和中脑 MPTP 损伤侧的铁水平之间没有关系。总之，这些结果证实了 SN 铁积累参与了 MPTP 处理的 PD 猴模型，并间接验证了 SWI 用于测量 PD 患者脑核中铁沉积的可用性。
