College of Materials and Metallurgy, Guizhou University, Guiyang, Guizhou, China.
Medical College, Guizhou University, Guiyang, Guizhou, China.
J Biomater Sci Polym Ed. 2020 Mar;31(4):439-455. doi: 10.1080/09205063.2019.1697171. Epub 2019 Dec 5.
Natural blood vessels have a multi-layered, cell-specific oriented spatial structure, mimicking of this structure is a promising way for blood vessel regeneration. In this study, a newly developed dual-oriented/bilayered small-diameter tubular scaffold was electrospun using a mixture of poly (ε-caprolactone) (PCL), poly (D, L-lactide-co-glycolide) (PLGA) and gelatin. The nanofiber orientations of the bilayers were spatially perpendicular to each other, aiming at guiding cell-specific orientation of smooth muscle cells (SMCs) and endothelial cells (ECs) respectively. The results showed that the hydrophilicity of scaffold was greatly improved by gelatin, and the mechanical property of this scaffold was the best among all. The degradation demonstrated that by mixing of three biodegradable polymers, a relatively fast degradation rate was achieved. After SMCs and ECs were seeded on scaffolds, cell viability, cellular morphology, and cytoskeleton behavior were investigated. The results revealed that as-electrospun scaffolds could promote both SMCs and ECs proliferation. Moreover, topographic cues offered by oriented nanofibers could guide the growth and orientation of SMCs and ECs. Therefore, the dual-oriented/bilayered electrospun scaffold is a superior structural and functional analogue to natural blood vessel and a potential candidate for vascular tissue engineering.
天然血管具有多层次、细胞特异性取向的空间结构，模拟这种结构是血管再生的一种有前途的方法。在这项研究中，使用聚（ε-己内酯）（PCL）、聚（D，L-乳酸-共-乙醇酸）（PLGA）和明胶的混合物，通过静电纺丝开发了一种新的双取向/双层小直径管状支架。双层的纳米纤维取向彼此空间垂直，旨在分别引导平滑肌细胞（SMCs）和内皮细胞（ECs）的细胞特异性取向。结果表明，明胶极大地提高了支架的亲水性，并且该支架的机械性能在所有支架中是最好的。降解表明，通过混合三种可生物降解聚合物，可以实现相对较快的降解速率。在将 SMCs 和 ECs 接种到支架上后，研究了细胞活力、细胞形态和细胞骨架行为。结果表明，静电纺丝支架可以促进 SMCs 和 ECs 的增殖。此外，取向纳米纤维提供的形貌线索可以指导 SMCs 和 ECs 的生长和取向。因此，双取向/双层静电纺丝支架是天然血管的优越结构和功能模拟物，是血管组织工程的潜在候选物。
