Architecture et Réactivité de l'ARN, Université de Strasbourg, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
Biochem Biophys Res Commun. 2019 Dec 17;520(4):702-704. doi: 10.1016/j.bbrc.2019.10.009.
In many RNA molecules, pseudouridines occur at conserved positions in functional sites. A great diversity of pseudouridine synthases guarantees the insertion of the modified base at precise locations. The accepted structural role of pseudouridines is a reduction of the RNA flexibility around the modification site. However, experiments rarely yield clear-cut evidence. The article "Dynamic stacking of an expected branch point adenosine in duplexes containing pseudouridine-modified or unmodified U2 snRNA sites" published in 2019 in Biochemical and Biophysical Research Communication by Kennedy et al. constitute a provocative case [1]. This example illustrates how a definite conformational state can be selected through small energy differences in a constrained environment.
在许多 RNA 分子中，假尿嘧啶核苷出现在功能位点的保守位置。大量的假尿嘧啶核苷合酶保证了修饰碱基精确地插入到特定位置。假尿嘧啶核苷被认为可以降低修饰位点附近 RNA 的灵活性。然而，实验很少能提供明确的证据。Kennedy 等人于 2019 年在《生物化学与生物物理研究通讯》发表的文章“含假尿嘧啶核苷修饰或未修饰 U2 snRNA 位点的双链体中预期分支点腺苷的动态堆积”[1]就是一个有启发性的例子。这个例子说明了在受限环境中，通过微小的能量差异如何选择确定的构象状态。
