剪接体小核 RNA 假尿嘧啶化的功能和机制。
Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation.
机构信息
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
出版信息
Wiley Interdiscip Rev RNA. 2011 Jul-Aug;2(4):571-81. doi: 10.1002/wrna.77. Epub 2011 Feb 18.
Abstract
Pseudouridines are the most abundant and highly conserved modified nucleotides identified in spliceosomal small nuclear RNAs (snRNAs). Most pseudouridines are also clustered in functionally important regions of spliceosomal snRNAs. Experiments carried out in several independent experimental systems show that the pseudouridines in spliceosomal snRNAs are functionally important for pre-messenger RNA (mRNA) splicing. Experimental data also indicate that spliceosomal snRNA pseudouridylation can be catalyzed by both RNA-dependent (box H/ACA Ribonucleoproteins) and RNA-independent (protein-only enzymes) mechanisms.
摘要
假尿嘧啶核苷是剪接体小核 RNA（snRNA）中含量最丰富和高度保守的修饰核苷酸。大多数假尿嘧啶核苷也聚集在剪接体 snRNA 的功能重要区域。在几个独立的实验系统中进行的实验表明，剪接体 snRNA 中的假尿嘧啶核苷对于前信使 RNA（mRNA）剪接具有重要的功能作用。实验数据还表明，剪接体 snRNA 的假尿嘧啶核苷化可以由 RNA 依赖性（框 H/ACA 核糖核蛋白）和 RNA 非依赖性（仅蛋白质酶）机制催化。
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