School of Mechanics and Engineering Science, Zhengzhou University, Zhengzhou, 450001, PR China; National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, 450001, PR China.
National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, 450001, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Feb;107:110212. doi: 10.1016/j.msec.2019.110212. Epub 2019 Oct 30.
A critical challenge to the development of tissue engineering small-diameter vascular grafts is to achieve rapid endothelialization and long-term anticoagulation. It is necessary to graft both adhesion and antithrombus factors onto the surface of polycaprolactone without burst release to promote endothelial cell affinity and antithrombogenicity. A bionic structure with a nanocoating that allows a biologically responsive, long-term release was employed in this work to enable the grafting of various bioactive molecules such as gelatin, polylysine, and heparin. This approach involved orienting the biomimetic vascular structures; the self-assembly grafting of gelatin, polylysine, and heparin nanoparticles; and genipin crosslinking to form a multiphase crosslinked nanocoating. In this biologically inspired design, vascular endothelialization and long-term anticoagulation were successfully induced through a matrix metallopeptidase 2 regulative mechanism by delivering both adhesion and antithrombus factors with a responsive, long-term release without burst release. The method provided a simple and effective approach for delivering dual factors for tissue engineering small-diameter vascular grafts.
组织工程小直径血管移植物发展的一个关键挑战是实现快速内皮化和长期抗凝。有必要在聚己内酯表面接枝既具有粘附性又具有抗血栓性的因子，而不会发生突释，从而促进内皮细胞的亲和性和抗血栓性。本工作采用具有纳米涂层的仿生结构，可实现生物响应性、长期释放，从而能够接枝各种生物活性分子，如明胶、聚赖氨酸和肝素。该方法涉及到仿生血管结构的取向、明胶、聚赖氨酸和肝素纳米颗粒的自组装接枝，以及京尼平交联形成多相交联纳米涂层。在这种受生物启发的设计中，通过基质金属蛋白酶 2 调节机制，成功地诱导了血管内皮化和长期抗凝，通过响应性、长期释放而没有突释来输送粘附和抗血栓因子。该方法为组织工程小直径血管移植物提供了一种简单有效的双重因子输送方法。
