Pai B Sujatha, Pradeep A R
Department of Periodontology, DSCDS.
Department of Periodontology, Government Dental College and Research Institute.
Bull Tokyo Dent Coll. 2019 Dec 10;60(4):279-289. doi: 10.2209/tdcpublication.2019-0002. Epub 2019 Nov 22.
Interleukin (IL)-33 is a dual-function protein that may play important roles as both a cytokine and intracellular nuclear factor. It may also function similarly to prototypical alarmin IL-1α, as an endogenous danger signal to alert innate immune system cells to tissue damage during trauma or infection, as it can be released in the extracellular space after endothelial cell damage or mechanical injury. The aim of this study was to determine possible correlations between concentrations of IL-33 and IL-1α in gingival crevicular fluid (GCF) and plasma obtained from healthy patients, those with chronic periodontitis (CP), and those with generalized aggressive periodontitis (GAP) patients. Forty-five patients with an age range of 20-60 years were enrolled and divided into 3 groups: Group H, 30 samples (15 GCF and 15 plasma) from 15 patients with healthy periodontium; Group CP, 30 samples from 15 patients with CP; and Group GAP, 30 samples from 15 patients with GAP. The clinical periodontal parameters investigated in all groups comprised the gingival index score, probing pocket depth, and clinical attachment level. The GCF and plasma levels of IL-33 and IL-1α were quantitated using enzyme linked immunosorbent assay. The mean IL-33 concentrations in GCF and plasma were highest in Group GAP, followed by Group CP, with the lowest in Group H; the difference among them was statistically significant (p<0.05). Concentration of IL-1α followed the same trend as that of IL-33 in GCF, but was lower than detection levels in plasma. The GCF and plasma concentrations of IL-33 correlated with IL-1α concentrations in GCF. Concentrations of IL-33 and IL-1α in GCF varied significantly between healthy patients and those with disease, allowing healthy patients to be distinguished from those with GAP or CP. The results of this study suggest that IL-33 offers a potential inflammatory marker of periodontal disease, similar to IL-1α.
白细胞介素（IL）-33是一种具有双重功能的蛋白质，它可能作为细胞因子和细胞内核因子发挥重要作用。它也可能与典型的警报素IL-1α功能相似，作为一种内源性危险信号，在创伤或感染期间提醒先天性免疫系统细胞注意组织损伤，因为它可以在内皮细胞损伤或机械损伤后释放到细胞外空间。本研究的目的是确定从健康患者、慢性牙周炎（CP）患者和广泛侵袭性牙周炎（GAP）患者获取的龈沟液（GCF）和血浆中IL-33和IL-1α浓度之间可能存在的相关性。招募了45名年龄在20至60岁之间的患者，并将其分为3组：H组，来自15名牙周健康患者的30份样本（15份GCF和15份血浆）；CP组，来自15名CP患者的30份样本；GAP组，来自15名GAP患者的30份样本。所有组中调查的临床牙周参数包括牙龈指数评分、探诊深度和临床附着水平。使用酶联免疫吸附测定法定量GCF和血浆中IL-33和IL-1α的水平。GCF和血浆中IL-33的平均浓度在GAP组中最高，其次是CP组，H组中最低；它们之间的差异具有统计学意义（p<0.05）。IL-1α在GCF中的浓度与IL-33的趋势相同，但低于血浆中的检测水平。GCF中IL-33和IL-1α的浓度与血浆中IL-1α的浓度相关。健康患者与患病患者之间GCF中IL-33和IL-1α的浓度差异显著，这使得健康患者能够与患有GAP或CP的患者区分开来。本研究结果表明，IL-33与IL-1α类似，是牙周疾病潜在的炎症标志物。
