Department of Periodontology, Faculty of Dentistry, Adnan Menderes University, Aydın, Turkey.
Department of Periodontology, Faculty of Dentistry, Ege University, İzmir, Turkey.
J Periodontol. 2019 Jul;90(7):788-797. doi: 10.1002/JPER.18-0412. Epub 2018 Dec 11.
Hypoxia-inducible factor-1 alpha (HIF-1α) is expressed as an adaptive response to hypoxia, mediates angiogenesis through the expression of vascular endothelial growth factor (VEGF) and can be induced by tumor necrosis factor-alpha (TNF-α). This study aimed to investigate the gingival crevicular fluid (GCF) and salivary HIF-1α, VEGF, and TNF-α levels in periodontal health and disease.
A total of 87 individuals, 20 generalized aggressive periodontitis (G-AgP), 20 chronic periodontitis (CP), 26 gingivitis patients, and 21 periodontally healthy individuals, were included. Clinical periodontal parameters were recorded; GCF and salivary samples were collected; and HIF-1α, VEGF, and TNF-α levels were measured by enzyme-linked immunosorbent assay. Nonparametric tests were used for the statistical analyses.
G-AgP and CP groups had significantly higher GCF HIF-1α, VEGF, and TNF-α total amounts than gingivitis and healthy groups (P < 0.05). GCF HIF-1α and TNF-α total amounts in gingivitis group were significantly higher than the healthy group (P < 0.05). GCF and salivary concentrations of biomarkers were similar in both periodontitis groups (P > 0.05). Salivary HIF-1α concentrations in gingivitis group were significantly higher than G-AgP and healthy groups (P < 0.05). GCF HIF-1α, VEGF, and TNF-α total amounts were positively correlated with the site-specific clinical periodontal parameters and with each other (P < 0.05).
HIF-1α is detectable in GCF and saliva of periodontally diseased and healthy individuals, and the GCF levels of the biomarker can be affected by disease status. Increased GCF HIF-1α, VEGF, and TNF-α levels in both chronic and aggressive form of periodontitis might suggest the role of TNF-α/HIF-1α/VEGF pathway in the pathogenesis of periodontal diseases.
缺氧诱导因子-1 阿尔法（HIF-1α）作为对缺氧的适应性反应而表达，通过血管内皮生长因子（VEGF）的表达介导血管生成，并且可以被肿瘤坏死因子-α（TNF-α）诱导。本研究旨在探讨牙周健康和疾病患者的龈沟液（GCF）和唾液中的 HIF-1α、VEGF 和 TNF-α 水平。
共纳入 87 名个体，其中 20 名广泛性侵袭性牙周炎（G-AgP）患者、20 名慢性牙周炎（CP）患者、26 名牙龈炎患者和 21 名牙周健康个体。记录临床牙周参数；采集 GCF 和唾液样本；并通过酶联免疫吸附试验测量 HIF-1α、VEGF 和 TNF-α 水平。采用非参数检验进行统计分析。
G-AgP 和 CP 组的 GCF HIF-1α、VEGF 和 TNF-α 总量明显高于牙龈炎和健康组（P<0.05）。牙龈炎组的 GCF HIF-1α 和 TNF-α 总量明显高于健康组（P<0.05）。两组牙周炎患者的 GCF 和唾液中生物标志物浓度相似（P>0.05）。牙龈炎组的唾液 HIF-1α 浓度明显高于 G-AgP 和健康组（P<0.05）。GCF HIF-1α、VEGF 和 TNF-α 总量与特定部位的临床牙周参数呈正相关，且彼此之间也呈正相关（P<0.05）。
HIF-1α可在牙周病患者和健康个体的 GCF 和唾液中检测到，且生物标志物的 GCF 水平可能受疾病状态的影响。慢性和侵袭性牙周炎中 GCF HIF-1α、VEGF 和 TNF-α 水平的增加可能提示 TNF-α/HIF-1α/VEGF 通路在牙周病发病机制中的作用。
