National Engineering Research Center of Biological Feed, Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing, China.
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
J Anim Physiol Anim Nutr (Berl). 2020 Jan;104(1):230-236. doi: 10.1111/jpn.13245. Epub 2019 Nov 24.
To investigate the effects of different levels of enzymatic hydrolysate of dietary locust bean gum on nutrient digestibility, intestinal morphology and microflora of broilers, a total of 768 one-day-old Arbor Acres (AA) broiler chicks were randomly divided into 6 treatments with 8 replicates per treatment and 16 birds per replicate. The treatments were as follows: (1) CON, basal diet; (2) ANT, basal diet +62.5 mg/kg flavomycin; (3) LBG, basal diet +0.1% locust bean gum; (4) ELBG-0.1, basal diet +0.1% enzymatic hydrolysate of LBG; (5) ELBG-0.2, basal diet +0.2% enzymatic hydrolysate of LBG; and (6) ELBG-0.3, basal diet +0.3% enzymatic hydrolysate of LBG. The digestibilities of ether extract, crude protein and dry matter were increased (p < .01) in broilers fed the ELBG-0.3 diet compared with the CON and LBG diets on day 21. Duodenal villus height and the ratio of the villus height to crypt depth were greater (p < .01) in broilers fed the ELBG-0.3 diet than the CON, ANT and LBG diets. Jejunum villus height was higher (p < .05) in broilers fed the ELBG-0.2 and ELBG-0.3 diets than the CON diet. The number of caecal Escherichia coli was reduced (p = .01) in broilers fed the ELBG-0.2 and ELBG-0.3 diets compared with the CON diet. The number of caecal Lactobacilli was greater (p < .05) in broilers fed the ELBG-0.3 diet than the CON and ANT diets. In summary, the addition of 0.3% locust bean enzymatic hydrolysate can increase the surface area of intestinal villi and the number of beneficial bacteria, inhibit the proliferation of harmful bacteria, maintain the balance of intestinal microflora and improve the digestibility of nutrients.
为了研究不同水平的槐豆胶酶解产物对肉鸡养分消化率、肠道形态和微生物区系的影响，将 768 只 1 日龄的爱拔益加（AA）肉鸡随机分为 6 个处理，每个处理 8 个重复，每个重复 16 只鸡。处理如下：（1）CON，基础日粮；（2）ANT，基础日粮+62.5mg/kg 金霉素；（3）LBG，基础日粮+0.1%槐豆胶；（4）ELBG-0.1，基础日粮+0.1%槐豆胶酶解产物；（5）ELBG-0.2，基础日粮+0.2%槐豆胶酶解产物；（6）ELBG-0.3，基础日粮+0.3%槐豆胶酶解产物。与 CON 和 LBG 日粮相比，第 21 天饲喂 ELBG-0.3 日粮的肉鸡的乙醚提取物、粗蛋白和干物质消化率提高（p<0.01）。饲喂 ELBG-0.3 日粮的肉鸡的十二指肠绒毛高度和绒毛高度与隐窝深度的比值大于 CON、ANT 和 LBG 日粮（p<0.01）。饲喂 ELBG-0.2 和 ELBG-0.3 日粮的肉鸡的空肠绒毛高度高于 CON 日粮（p<0.05）。与 CON 日粮相比，饲喂 ELBG-0.2 和 ELBG-0.3 日粮的肉鸡的盲肠大肠杆菌数量减少（p=0.01）。饲喂 ELBG-0.3 日粮的肉鸡的盲肠乳酸杆菌数量多于 CON 和 ANT 日粮（p<0.05）。综上所述，添加 0.3%的槐豆胶酶解产物可以增加肠道绒毛的表面积和有益菌的数量，抑制有害菌的增殖，维持肠道微生物区系的平衡，提高养分的消化率。
