Jiang Kangjia, Wang Duo, Su Le, Liu Xinli, Yue Qiulin, Li Baojun, Li Kunlun, Zhang Song, Zhao Lin
State Key Laboratory of Bio-Based Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China.
Shandong Zhuoran Biotechnology Co., Ltd., Jinan, China.
Front Microbiol. 2022 Aug 10;13:985725. doi: 10.3389/fmicb.2022.985725. eCollection 2022.
Ulcerative colitis (UC) is an inflammatory lesion of the colon from various causes. As current therapeutic drugs have adverse effects on patients with UC, there is a growing demand for alternative medicines from natural and functional foods. Locust bean gum, as a dietary fiber, has a variety of physiological effects.
In the present study, locust bean gum hydrolysate (LBGH) was obtained from the acid hydrolysis of locust bean gum. The structure of LBGH was characterized by thin-layer chromatography and high performance liquid chromatography (HPLC)-electrospray ionization (ESI)-mass spectrometry (MS)/MS analysis. And we investigated the therapeutic effect of LBGH on a mouse model of dextran sulfate sodium (DSS)-induced colitis.
It was observed that the LBGH consisted of a mixture of monosaccharides and oligosaccharides with a degree of polymerization (DP) 2-7. LBGH treatment dramatically alleviated colonic pathological damage, suppressed the overproduction of pro-inflammatory factors and the activation of nuclear factor κB (NF-κB), increased the mRNA expression of tight junction proteins, and increased the abundance of probiotics such as and in the gut.
There is a correlation between these mitigating effects on inflammation and the treatment of LBGH. Therefore, LBGH has tremendous potential in the treatment of colitis.
溃疡性结肠炎（UC）是由多种原因引起的结肠炎症性病变。由于目前的治疗药物对UC患者有不良反应，对天然和功能性食品中的替代药物的需求日益增加。刺槐豆胶作为一种膳食纤维，具有多种生理作用。
在本研究中，刺槐豆胶水解物（LBGH）通过刺槐豆胶的酸水解获得。LBGH的结构通过薄层色谱和高效液相色谱（HPLC）-电喷雾电离（ESI）-质谱（MS）/MS分析进行表征。并且我们研究了LBGH对葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型的治疗效果。
观察到LBGH由单糖和低聚糖的混合物组成，聚合度（DP）为2-7。LBGH治疗显著减轻结肠病理损伤，抑制促炎因子的过度产生和核因子κB（NF-κB）的激活，增加紧密连接蛋白的mRNA表达，并增加肠道中如 和 等益生菌的丰度。
这些对炎症的缓解作用与LBGH的治疗之间存在相关性。因此，LBGH在结肠炎治疗中具有巨大潜力。
