Lin Maohui, Ge Jinbo, Wang Xuecen, Dong Ziqing, Xing Malcolm, Lu Feng, He Yunfan
Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
Departments of Mechanical Engineering, and Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada.
J Tissue Eng. 2019 Nov 14;10:2041731419888168. doi: 10.1177/2041731419888168. eCollection 2019 Jan-Dec.
Decellularized adipose tissue (DAT) is a promising biomaterial for adipose tissue engineering. However, there is a lack of research of DAT prepared from xenogeneic porcine adipose tissue. This study aimed to compare the adipogenic ability of DAT derived from porcine subcutaneous (SDAT) and visceral adipose tissue (VDAT). The retention of key collagen in decellularized matrix was analysed to study the biochemical properties of SDAT and VDAT. For the biomechanical study, both DAT materials were fabricated into three-dimensional (3D) porous scaffolds for rheology and compressive tests. Human adipose-derived stem cells (ADSCs) were cultured on both scaffolds to further investigate the effect of matrix stiffness on cellular morphology and on adipogenic differentiation. ADSCs cultured on soft VDAT exhibited significantly reduced cellular area and upregulated adipogenic markers compared to those cultured on SDAT. In vivo results revealed higher adipose regeneration in the VDAT compared to the SDAT. This study further demonstrated that the relative expression of collagen IV and laminin was significantly higher in VDAT than in SDAT, while the collagen I expression and matrix stiffness of SDAT was significantly higher in comparison to VDAT. This result suggested that porcine adipose tissue could serve as a promising candidate for preparing DAT.
去细胞脂肪组织（DAT）是一种用于脂肪组织工程的有前景的生物材料。然而，对于由异种猪脂肪组织制备的DAT的研究较少。本研究旨在比较源自猪皮下脂肪组织（SDAT）和内脏脂肪组织（VDAT）的DAT的成脂能力。分析去细胞基质中关键胶原蛋白的保留情况，以研究SDAT和VDAT的生化特性。为进行生物力学研究，将两种DAT材料制成三维（3D）多孔支架进行流变学和压缩测试。将人脂肪来源干细胞（ADSCs）培养在两种支架上，以进一步研究基质硬度对细胞形态和成脂分化的影响。与培养在SDAT上的细胞相比，培养在柔软的VDAT上的ADSCs细胞面积显著减小，成脂标志物上调。体内结果显示，与SDAT相比，VDAT中的脂肪再生更高。本研究进一步表明，IV型胶原蛋白和层粘连蛋白在VDAT中的相对表达明显高于SDAT，而SDAT中的I型胶原蛋白表达和基质硬度明显高于VDAT。该结果表明，猪脂肪组织可作为制备DAT的有前景的候选材料。
