State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
J Biomed Mater Res A. 2018 Sep;106(9):2563-2574. doi: 10.1002/jbm.a.36435. Epub 2018 May 14.
Natural and synthetic materials have been developed to provide a three-dimensional (3-D) scaffold that mimics the extracellular matrix properties for tissue regeneration and reconstruction. On account of the excellent biological and mechanical properties, diverse decellularized tissue materials have been developed as natural scaffolds. Among them, decellularized adipose tissue (DAT) has generated great interest in recent years due to the extensiveness of the source and the eximious potential to enhance the regeneration and repair of damaged tissues. In this review, we compared the different physical, chemical, and biological treatment in recorded decellularization methods, and illustrated the similarities and differences in the characteristics of various aspects of DATs prepared by different methods, including macroscopic (macro) and microcosmic (micro) structure, cellular contents removal, DNA quantification, collagen, glycosaminoglycan (GAG) or growth factors retention, and mechanical properties. We also summarized the DAT application in the studies of tissue regeneration, wound healing, drug screening, and other diseases. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2562-2573, 2018.
天然材料和合成材料已被开发出来，以提供一种模仿细胞外基质特性的三维（3-D）支架，用于组织再生和重建。由于具有优异的生物和机械性能，各种脱细胞组织材料已被开发为天然支架。其中，由于来源广泛，并且具有增强受损组织再生和修复的巨大潜力，脱细胞脂肪组织（DAT）近年来引起了极大的兴趣。在本综述中，我们比较了记录的脱细胞化方法中的不同物理、化学和生物学处理，并说明了不同方法制备的 DAT 在宏观和微观结构、细胞内容物去除、DNA 定量、胶原、糖胺聚糖（GAG）或生长因子保留以及机械性能等各个方面的特征的异同。我们还总结了 DAT 在组织再生、伤口愈合、药物筛选和其他疾病研究中的应用。© 2018 Wiley Periodicals, Inc. J 生物材料研究杂志 A 部分：106A:2562-2573, 2018.
