Lu Hongyun, Ying Keqin, Shi Ying, Liu Donghong, Chen Qihe
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
College of Food Science and Technology, Nanjing University, Nanjing 210095, China.
Bioengineering (Basel). 2022 Dec 9;9(12):787. doi: 10.3390/bioengineering9120787.
As novel carrier biomaterials, decellularized scaffolds have promising potential in the development of cellular agriculture and edible cell-cultured meat applications. Decellularized scaffold biomaterials have characteristics of high biocompatibility, bio-degradation, biological safety and various bioactivities, which could potentially compensate for the shortcomings of synthetic bio-scaffold materials. They can provide suitable microstructure and mechanical support for cell adhesion, differentiation and proliferation. To our best knowledge, the preparation and application of plant and animal decellularized scaffolds have not been summarized. Herein, a comprehensive presentation of the principles, preparation methods and application progress of animal-derived and plant-derived decellularized scaffolds has been reported in detail. Additionally, their application in the culture of skeletal muscle, fat and connective tissue, which constitute the main components of edible cultured meat, have also been generally discussed. We also illustrate the potential applications and prospects of decellularized scaffold materials in future foods. This review of cultured meat and decellularized scaffold biomaterials provides new insight and great potential research prospects in food application and cellular agriculture.
作为新型载体生物材料，脱细胞支架在细胞农业和食用细胞培养肉应用的发展中具有广阔的潜力。脱细胞支架生物材料具有高生物相容性、生物降解性、生物安全性和多种生物活性等特点，有可能弥补合成生物支架材料的缺点。它们可以为细胞的黏附、分化和增殖提供合适的微观结构和机械支持。据我们所知，动植物脱细胞支架的制备和应用尚未得到总结。在此，详细报道了动物源和植物源脱细胞支架的原理、制备方法及应用进展。此外，还对它们在构成食用培养肉主要成分的骨骼肌、脂肪和结缔组织培养中的应用进行了一般性讨论。我们还阐述了脱细胞支架材料在未来食品中的潜在应用和前景。这篇关于培养肉和脱细胞支架生物材料的综述为食品应用和细胞农业提供了新的见解和巨大的潜在研究前景。
