Uvnäs-Wallensten K
Acta Physiol Scand. 1978 Dec;104(4):386-93. doi: 10.1111/j.1748-1716.1978.tb06293.x.
In acute experiments on cats antral pouches were perfused with solutions of different pH (1-13). After antrum passage the gastrin levels in the perfusates were measured with radioimmunoassay and the amounts of gastrin released into the antral lumen per minute calculated. The venous gastrin levels were determined concomitantly. Small amounts of gastrin (1,000--1,500 pg/min) were released into the antrum during perfusion with 0.1 M HCl. Subsequent perfusion with 0.15 M NaCl (pH 6.8) did not significantly increase the release of gastrin. On the other hand, 0.1 M phosphate buffer (pH 7.4) caused a dramatic augmentation of the gastrin output into the antral lumen (approximately 17 fold). A concomitant increase of peripheral gastrin levels was observed. Also other alkaline solutions such as 0.15 M NaHCO3 (pH 8), 0.15 M Tris buffer (pH) or 0.01 and 0.1 M NaOH (pH 12 and 13) promoted the release of gastrin. It is discussed whether the gastrin release at alkaline pH is induced by the alkaline pH itself or by anions such as HPO-4, HCO-3 and OH-. The apparent effect of pH could then be due to the formation of these ions at higher pH.
在对猫进行的急性实验中，用不同pH值（1 - 13）的溶液灌注胃窦袋。胃窦通过后，用放射免疫分析法测量灌注液中的胃泌素水平，并计算每分钟释放到胃窦腔内的胃泌素量。同时测定静脉血中的胃泌素水平。在用0.1 M盐酸灌注期间，少量胃泌素（1000 - 1500 pg/分钟）释放到胃窦中。随后用0.15 M氯化钠（pH 6.8）灌注并未显著增加胃泌素的释放。另一方面，0.1 M磷酸盐缓冲液（pH 7.4）使进入胃窦腔的胃泌素输出量急剧增加（约17倍）。同时观察到外周血胃泌素水平升高。其他碱性溶液如0.15 M碳酸氢钠（pH 8）、0.15 M Tris缓冲液（pH）或0.01和0.1 M氢氧化钠（pH 12和13）也促进胃泌素的释放。讨论了碱性pH值下胃泌素的释放是由碱性pH值本身还是由诸如HPO₄²⁻、HCO₃⁻和OH⁻等阴离子诱导的。那么pH值的明显作用可能是由于在较高pH值下这些离子的形成。
