Zeyad Mohammad Tarique, Kumar Murugan, Malik Abdul
Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, UP-202002, India.
National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau, UP-275103, India.
Biotechnol Rep (Amst). 2019 Oct 24;24:e00389. doi: 10.1016/j.btre.2019.e00389. eCollection 2019 Dec.
Atomic absorption spectrophotometer and gas chromatography analysis revealed the presence of heavy metals, organochlorine and organophosphate pesticides in industrial wastewater. XAD, Dichloromethane and n-Hexane extracted wastewater were analysed for genotoxic potential using Ames /mammalian microsome test. The XAD concentrated sample displayed remarkable mutagenic activity compared to solvent assisted liquid-liquid extraction. Strain TA98 was found utmost sensitive towards all extracts. Wastewater induced chromosomal aberrations in roots of showed significant ( < 0.05) decrease in mitotic index. Seeds of germinated on soft agar plates treated with different concentration of wastewater showed significant reduction in germination (52 %), seedling vigor index (76 %), radicle length (56 %), plumule length (47 %), biomass of radicle (64 %) and plumule (57%) at highest wastewater concentration. Propidium iodide stained roots showed oxidative stress induced by wastewater under CLS microscopy. Further, genotoxicity of wastewater was confirmed by plasmid nicking assay using pBR322 plasmid.
原子吸收分光光度计和气相色谱分析表明，工业废水中存在重金属、有机氯和有机磷农药。使用艾姆斯/哺乳动物微粒体试验对经XAD、二氯甲烷和正己烷萃取的废水的遗传毒性潜力进行了分析。与溶剂辅助液-液萃取相比，XAD浓缩样品显示出显著的诱变活性。发现菌株TA98对所有提取物最为敏感。废水诱导的根染色体畸变显示有丝分裂指数显著（<0.05）下降。在经不同浓度废水处理的软琼脂平板上萌发的种子，在最高废水浓度下，发芽率（52%）、幼苗活力指数（76%）、胚根长度（56%）、胚芽长度（47%）、胚根生物量（64%）和胚芽生物量（57%）均显著降低。碘化丙啶染色的根在共聚焦激光扫描显微镜下显示废水诱导了氧化应激。此外，使用pBR322质粒的质粒切口试验证实了废水的遗传毒性。
