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利用细菌和植物生物测定法评估纺织工业废水的致突变性和遗传毒性。

Mutagenicity and genotoxicity evaluation of textile industry wastewater using bacterial and plant bioassays.

作者信息

Khan Sana, Anas Mohammad, Malik Abdul

机构信息

Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.

出版信息

Toxicol Rep. 2019 Feb 15;6:193-201. doi: 10.1016/j.toxrep.2019.02.002. eCollection 2019.

Abstract

Textile industrial wastewater samples were taken from the Panki site 5 industrial area of Kanpur city, India. Atomic Absorption spectrophotometer and Gas Chromatography-Mass spectrometry techniques have shown that the wastewater contained several heavy metals and organic pollutants (Khan and Malik, 2017) [1]. Further, in order to explore the potential toxicity of these pollutants present in the effluent, a battery of short-term biological assays (Ames test, DNA repair defective mutation assay and chromosomal aberration test) were used. Wastewater samples were concentrated with XAD-4/8 resins and liquid-liquid extraction procedure. XAD-concentrated samples were more mutagenic than the liquid-liquid extracted samples. Ames TA98 and A (SOS defective) strains were the most responsive strains. The wastewater also resulted in significant decline in mitotic index and induced chromosomal aberrations in . roots. The findings thus showed that the combination of physico-chemical analysis alongwith the toxicity assessment (using short term biological assays) would provide valuable and more realistic information about the joint toxicity of chemical pollutants present in the textile effluent.

摘要

纺织工业废水样本取自印度坎普尔市潘基5号工业区。原子吸收分光光度计和气相色谱 - 质谱技术表明,该废水中含有多种重金属和有机污染物(汗和马利克,2017年)[1]。此外,为了探究这些废水中污染物的潜在毒性,采用了一系列短期生物测定法(艾姆斯试验、DNA修复缺陷突变试验和染色体畸变试验)。废水样本通过XAD - 4/8树脂和液 - 液萃取程序进行浓缩。XAD浓缩样本比液 - 液萃取样本具有更强的致突变性。艾姆斯TA98和A(SOS缺陷)菌株是最敏感的菌株。该废水还导致有丝分裂指数显著下降,并在根中诱导染色体畸变。因此,研究结果表明,物理化学分析与毒性评估(使用短期生物测定法)相结合,将为纺织废水中化学污染物的联合毒性提供有价值且更现实的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd98/6396094/09c7f30cfb44/gr1.jpg

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