Faculté des Sciences de la Nature et de la Vie et des Sciences de la Terre et de l'Univers, Université 8 Mai 1945 Guelma, BP 401, 24000, Guelma, Algeria.
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Üsküdar University, Altunizade, 34662, Istanbul, Turkey.
Environ Sci Pollut Res Int. 2019 Jun;26(18):18403-18410. doi: 10.1007/s11356-019-05225-8. Epub 2019 May 2.
Nanoparticles are very effective compounds to transform and detoxicate common environmental contaminants. For this reason, crude urban liquid wastewater sludges were treated by silver nanoparticles (Ag-NPs, 100 nm) for 24 h. Both Ag-NPs' treated and untreated sludges were examined for the evaluation if there are possible mutagenic/anti-mutagenic, cytotoxic, and genotoxic/anti-genotoxic effects by Ames and Allium cepa tests. The results were then subjected to statistical analyses by using SPSS software and p < 0.05 was accepted as a significant value. The data obtained from the Ames test showed that while untreated crude liquid sludge had a significant mutagenic effect, Ag-NP-treated one decreased its mutagenicity. Similar effects were also observed in the chromosome aberration-Allium cepa tests. Significant chromosome aberrations observed were C-metaphase, sticky metaphase, sticky anaphase, anaphase bridge, vagrant chromosome, and multipolar anaphases. Both tests demonstrated that silver nanoparticle treatment decreased the major mutagenicity and genotoxicity detected in the liquid wastewater sludges.
纳米颗粒是将常见环境污染物转化和解毒的非常有效的化合物。因此，将粗制城市液态废水污泥用银纳米颗粒（Ag-NPs，100nm）处理 24 小时。用 Ames 和洋葱实验检测 Ag-NPs 处理和未处理的污泥是否具有可能的诱变/抗诱变、细胞毒性和遗传毒性/抗遗传毒性作用。然后使用 SPSS 软件对结果进行统计分析，p<0.05 被认为是显著值。Ames 试验获得的数据表明，未经处理的粗液态污泥具有显著的诱变作用，而 Ag-NP 处理则降低了其诱变作用。在染色体畸变-洋葱实验中也观察到了类似的效果。观察到的显著染色体畸变包括 C-中期、粘性中期、粘性后期、后期桥、游移染色体和多极后期。这两个实验都表明，银纳米颗粒处理降低了在液态废水污泥中检测到的主要诱变和遗传毒性。
