Hoseindoost Maryam, Alipour Mohammad Reza, Farajdokht Fereshteh, Diba Roghayeh, Bayandor Parvin, Mehri Keyvan, Nayebi Rad Sepehr, Babri Shirin
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Avicenna J Phytomed. 2019 Nov-Dec;9(6):597-605. doi: 10.22038/AJP.2019.13587.
In the present study, we aimed to examine the effect of troxerutin treatment on levels of brain-derived neurotrophic factor (BDNF), and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), as pro-inflammatory cytokines, in the blood and hippocampus of high-fat diet (HFD) fed dams and their male offspring.
Forty virgin female Wistar rats, 3 weeks old, were divided into two groups (n=20/per group) and fed control diet (CD), or HFD for 8 weeks. After mating, pregnant animals were assigned to four subgroups including: control (CD), control+troxerutin (CD+T), high-fat diet (HFD), and HFD+troxerutin (HFD+T) groups. HFD was continued during pregnancy and lactation. Troxerutin (150 mg/kg/day, P.O.) was administered during pregnancy in the CD+T and HFD+T groups. On postnatal day (PND) 21, male offspring were separated and fed a normal diet until PND 90. Inflammatory cytokines (TNF-α, and IL-6) and BDNF levels were measured in the serum and hippocampal samples using rat-specific enzyme-linked immunosorbent assay (ELISA) kits.
Our findings showed a significant increase in the serum levels of TNF-α and IL-6, but a decrease in BDNF levels in the serum of HFD-fed dams in comparison with CD group, which was reversed by troxerutin. Moreover, troxerutin treatment, during pregnancy, significantly decreased TNF-α and IL-6 levels, but increased BDNF level in the serum and hippocampus of HFD+T offspring in comparison with HFD offspring.
These results showed that troxerutin could prevent the harmful effects of maternal HFD on their offspring through inhibition of pro-inflammatory cytokines and elevation of BDNF levels.
在本研究中，我们旨在检测曲克芦丁治疗对高脂饮食（HFD）喂养的母鼠及其雄性后代血液和海马中脑源性神经营养因子（BDNF）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6，作为促炎细胞因子）水平的影响。
将40只3周龄的未孕雌性Wistar大鼠分为两组（每组n = 20），分别给予对照饮食（CD）或HFD，持续8周。交配后，将怀孕动物分为四个亚组，包括：对照组（CD）、对照+曲克芦丁组（CD+T）、高脂饮食组（HFD）和高脂饮食+曲克芦丁组（HFD+T）。孕期和哺乳期持续给予HFD。CD+T组和HFD+T组在孕期给予曲克芦丁（150 mg/kg/天，口服）。在出生后第21天（PND 21），将雄性后代分开，给予正常饮食直至PND 90。使用大鼠特异性酶联免疫吸附测定（ELISA）试剂盒测量血清和海马样本中的炎性细胞因子（TNF-α和IL-6）以及BDNF水平。
我们的研究结果显示，与CD组相比，HFD喂养的母鼠血清中TNF-α和IL-6水平显著升高，但BDNF水平降低，而曲克芦丁可逆转这种情况。此外，与HFD后代相比，孕期给予曲克芦丁治疗可显著降低HFD+T后代血清和海马中的TNF-α和IL-6水平，但提高BDNF水平。
这些结果表明，曲克芦丁可通过抑制促炎细胞因子和提高BDNF水平来预防母体HFD对其后代的有害影响。
