Children's Hospital of Fudan University, Shanghai, China.
Shanghai Key Laboratory of Birth Defect, Shanghai, China.
BMJ Open. 2019 Nov 24;9(11):e031076. doi: 10.1136/bmjopen-2019-031076.
The Shanghai Preconception Cohort (SPCC) was initially established to investigate the associations of parental periconceptional nutritional factors with congenital heart disease (CHD) but has further analysed child growth and development and paediatric diseases.
Preparing-for-pregnancy couples who presented at Shanghai preconception examination clinics and early-pregnancy women before 14 gestational weeks were enrolled to comprise the periconceptional baseline study population. General characteristics, routine clinical data and consumption of diet supplements, such as folic acid and multivitamins, were collected. Blood samples were obtained at preconception and early, middle and late gestations using standard procedures. Multiple nutritional factors, including folate, homocysteine, vitamin A, vitamin D, vitamin E and metals, in the blood samples of participants selected using a case-control design were examined. Genomic DNA was extracted.
The baseline population included 8045 preconception couples, 3054 single women and 15 615 early-pregnancy women. Data from 12 402 births were collected, and follow-up of the cohort for other outcomes is ongoing. Currently, 151 cases of CHD were identified after birth. The pilot analysis in a small subgroup showed that approximately 20.0% of preconception women and 44.9% of early-pregnancy women had red blood cell (RBC) folate levels that met the international recommendation for preventing neural tube defects.
Once a sufficient number of CHD cases are achieved, we will investigate the quantitative association of preconception RBC folate levels with CHD using a nested case-control design. The SPCC will be followed up for 18 years to investigate extensive outcomes of growth, development, obesity, and common and rare diseases during childhood and adolescence according to our plan. Blood nutritional factors will be examined in participants selected for specific aims. The SPCC will also allow for prospective cohort studies on extensive research questions.
NCT02737644.
上海孕前队列（SPCC）最初成立是为了研究父母围孕期营养因素与先天性心脏病（CHD）的关系，但后来进一步分析了儿童生长发育和儿科疾病。
在上海孕前检查诊所就诊的备孕夫妇和妊娠 14 周前的早孕妇女被纳入围孕期基线研究人群。收集一般特征、常规临床数据和饮食补充剂（如叶酸和多种维生素）的消费情况。采用标准程序在孕前、孕早期、孕中期和孕晚期采集参与者的血样。使用病例对照设计选择参与者的血液样本中检测了多种营养因素，包括叶酸、同型半胱氨酸、维生素 A、维生素 D、维生素 E 和金属。提取基因组 DNA。
基线人群包括 8045 对备孕夫妇、3054 名单身女性和 15615 名早孕妇女。收集了 12402 例分娩的数据，对该队列的其他结局的随访仍在进行中。目前，出生后发现 151 例 CHD 病例。小亚组的初步分析表明，大约 20.0%的备孕妇女和 44.9%的早孕妇女的红细胞（RBC）叶酸水平符合预防神经管缺陷的国际建议。
一旦获得足够数量的 CHD 病例，我们将使用巢式病例对照设计调查孕前 RBC 叶酸水平与 CHD 的定量关联。根据我们的计划，SPCC 将随访 18 年，以调查儿童和青少年时期生长、发育、肥胖以及常见和罕见疾病的广泛结局。将根据具体目标选择参与者检查血液营养因素。SPCC 还将允许进行广泛研究问题的前瞻性队列研究。
NCT02737644。
