Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, Shaanxi University of Chinese Medicine, Xian Yang, China.
J Med Food. 2020 Feb;23(2):125-131. doi: 10.1089/jmf.2019.4414. Epub 2019 Nov 26.
Polysaccharide from Ma-chi-xian ( L., POLP) was prepared and the therapeutic effect on dextran sodium sulfate-induced colitis mice was investigated in this study. The results of clinical activity score and H&E staining confirmed the therapeutic effect of POLP. POLP could diminished the symptoms of colitis and improve colon histopathological structure of the colitis mice. The expression levels of four cytokines were determined. The concentrations of PGE and IL-6 were downregulated by POLP treatment. The COX-2 protein expression levels and the STAT3 phosphorylation levels were detected. The results showed that these two protein levels were all increased in colitis and decreased after POLP treatment, indicating that these two proteins were closely related with the protective effect of POLP. Because the synthesis of PGE is catalyzed by COX-2 and phosphorylation of STAT3 can induce the expression of COX-2, it was concluded that STAT3 was a key protein related to the POLP exerting its activity in colitis.
本研究制备了麻芪消痔方（L.，POLP）多糖，并考察了其对葡聚糖硫酸钠诱导的结肠炎小鼠的治疗作用。临床活动评分和 H&E 染色结果证实了 POLP 的治疗效果。POLP 可减轻结肠炎症状，改善结肠炎小鼠结肠组织病理学结构。测定了四种细胞因子的表达水平。POLP 治疗可下调 PGE 和 IL-6 的浓度。检测 COX-2 蛋白表达水平和 STAT3 磷酸化水平。结果表明，这两种蛋白水平在结肠炎中均升高，POLP 治疗后降低，表明这两种蛋白与 POLP 的保护作用密切相关。由于 PGE 的合成由 COX-2 催化，STAT3 的磷酸化可诱导 COX-2 的表达，因此可以得出结论，STAT3 是与 POLP 在结肠炎中发挥作用相关的关键蛋白。
