Wang Zheng, Wang Li, Wu Xue, Pan Ya-Lei, Xie Pei, Pei Gang, Liang Yan-Ni, Tang Zhi-Shu, Liu Li
Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi Rheumatism and Tumor Center of TCM Engineering Technology Research, Shaanxi University of Chinese Medicine Xianyang 712083, China.
School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, Hunan, China.
Am J Transl Res. 2018 Aug 15;10(8):2502-2510. eCollection 2018.
(POL) is a traditional Chinese medicine and also an edible vegetable used to treat diarrhea in china for thousands years. Though the therapeutic effect has been proved in clinical trials, the concrete effective component and mechanisms remained elusive. Polysaccharide from POL has been extracted previously and the experiment suggested that POLP could diminish the weight loss and improve the health conditions of mice with DSS induced colitis. Hematoxylin & eosin staining revealed that POLP could improve the histopathological structure of the colon tissue. For the notably variation curve of TNF-α in control, colitis and treatment group, NF-κB was enrolled to investigate the molecular mechanisms of the protective effect of POLP. The protein expression level of NF-κBp65 in cytoplasm increased after POLP treatment of the induced colitis. However, the protein level of NF-κBp65 in the nucleus decreased after administration of POLP. The expression levels of IκBα and NF-κB related proteins Bcl-2 and survivin were also detected and the results suggested that POLP could inhibit the degradation of IκBα and decrease the protein levels of Bcl-2 and Survivin in colitis. It was concluded that POLP could improve the health condition of mice with DSS induced colitis and the mechanisms were closely related with NF-κB via inhibiting the degradation of IκBα.
马齿苋是一种传统中药，也是一种可食用蔬菜，在中国用于治疗腹泻已有数千年历史。尽管其治疗效果已在临床试验中得到证实，但其具体有效成分和作用机制仍不清楚。此前已从马齿苋中提取出多糖，实验表明马齿苋多糖可减轻葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的体重减轻并改善其健康状况。苏木精-伊红染色显示马齿苋多糖可改善结肠组织的组织病理学结构。针对对照组、结肠炎组和治疗组中肿瘤坏死因子-α（TNF-α）的显著变化曲线，研究了核因子-κB（NF-κB）以探讨马齿苋多糖保护作用的分子机制。马齿苋多糖处理诱导性结肠炎后，细胞质中NF-κBp65的蛋白表达水平升高。然而，给予马齿苋多糖后，细胞核中NF-κBp65的蛋白水平降低。还检测了IκBα以及与NF-κB相关的蛋白Bcl-2和生存素的表达水平，结果表明马齿苋多糖可抑制IκBα的降解，并降低结肠炎中Bcl-2和生存素的蛋白水平。得出的结论是，马齿苋多糖可改善DSS诱导的结肠炎小鼠的健康状况，其机制与NF-κB密切相关，通过抑制IκBα的降解来实现。
