MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, and College of Biophotonics , South China Normal University , Guangzhou 510631 , P.R. China.
J Am Chem Soc. 2019 Dec 11;141(49):19226-19230. doi: 10.1021/jacs.9b10353. Epub 2019 Dec 3.
Here, we report a reversible photoacoustic (PA) probe, BDP-DOH, to image the localized redox state in vivo via monitoring the dynamic changes of the redox couple, superoxide anion (O) and glutathione (GSH). The probe features a significant shift in absorption between 680 and 750 nm during selective oxidation with O and the reductive recovery of GSH, thus showing a reversible responsiveness in the photoacoustic intensity ratio (PA/PA) to successfully visualize the redox status of the tissue in situ. This redox probe with specific and sensitive photoacoustic response provides a potential technical tool toward understanding pathological formation processes.
在这里，我们报告了一种可逆的光声（PA）探针 BDP-DOH，通过监测氧化还原偶对超氧阴离子（O）和谷胱甘肽（GSH）的动态变化，来对体内局部的氧化还原状态进行成像。该探针在与 O 选择性氧化时，其吸收在 680nm 至 750nm 之间发生显著位移，而当 GSH 被还原时，吸收则恢复，因此在光声强度比（PA/PA）上表现出了可逆的响应性，成功地对组织的氧化还原状态进行了原位可视化。这种具有特定且灵敏的光声响应的氧化还原探针为理解病理形成过程提供了一种有潜力的技术工具。
