School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
School of Veterinary Medicine, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil.
Cryobiology. 2020 Feb 1;92:103-108. doi: 10.1016/j.cryobiol.2019.11.042. Epub 2019 Nov 23.
Cryopreservation of cats epididymal spermatozoa allows the conservation of the genetic material and the study of the cryogenic effect applied to the gametes of other felines. However, this biotechnique still presents variable results, being necessary the investigation of alternative extenders. Powdered coconut water (ACP-117c) has been efficient in the sperm freezing of several species and in the cat sperm refrigeration. Therefore, we aimed to evaluate the effect of the freezing stages and the quality of the cats' epididymal spermatozoa after thawing, using ACP-117c. Epididymides (n = 36) from 18 cats were processed using TRIS (n = 18) or ACP-117c (n = 18) for sperm recovery. The sperm were immediately evaluated. Then, this was cooled, glycerolized, frozen and thawed, and re-evaluated at each stage for sperm kinetics by Computer Assisted Semen Analysis, viability, functionality (HOST), mitochondrial activity (DAB) and morphology. There was a reduction in total motility and progressive motility after thawing in both groups, and TRIS was superior to ACP-117c. The curvilinear velocity reduced after thawing with ACP-117c. Viability decreased after glycerolization in TRIS. Although it also reduced after thawing in both groups, it was higher in TRIS. There was no change on HOST. Mitochondrial activity decreased during the cryopreservation steps for both extenders. Nevertheless, TRIS presented a higher percentage of spermatozoa from DAB class I and II after thawing. Morphology did not differ between extenders. Therefore, ACP-117c is an alternative for the recovery of cat epididymal spermatozoa; however, it is not efficient for freezing. Glycerolization and thawing are the most critical stages, regardless of the extender.
猫附睾精子的冷冻保存允许遗传物质的保存，并研究应用于其他猫科动物配子的低温效应。然而，这项生物技术仍然存在不同的结果，有必要调查替代的扩展剂。粉末状椰子水（ACP-117c）在几种物种的精子冷冻和猫精子冷藏中都很有效。因此，我们旨在使用 ACP-117c 评估冷冻阶段和解冻后猫附睾精子质量的影响。从 18 只猫的附睾中处理了 TRIS（n=18）或 ACP-117c（n=18）以回收精子。精子立即进行评估。然后，将其冷却、甘油化、冷冻和解冻，并在每个阶段重新评估精子动力学（计算机辅助精液分析）、活力、功能（HOST）、线粒体活性（DAB）和形态。在两组中，解冻后总活力和前向运动精子活力均下降，TRIS 优于 ACP-117c。解冻后，ACP-117c 的曲线速度下降。TRIS 中的甘油化后活力下降。尽管在两组中解冻后活力也下降，但 TRIS 中的活力更高。HOST 没有变化。两种扩展剂在冷冻保存步骤中都降低了线粒体活性。然而，解冻后 TRIS 中具有 DAB 类 I 和 II 的精子比例更高。形态在两种扩展剂之间没有差异。因此，ACP-117c 是回收猫附睾精子的一种替代方法；然而，它不适合冷冻。甘油化和解冻是最关键的阶段，与扩展剂无关。
