Habu S, Hayakawa K, Okumura K, Tada T
Eur J Immunol. 1979 Dec;9(12):938-42. doi: 10.1002/eji.1830091206.
Rabbit antiserum against mouse brain tissue (anti-brain-associated T cell antigen, anti-BAT) was capable of killing splenic natural killer (NK) cells of CBA/J, BALB/c, C 57 Bl/6J, C 3 H/He and nude mice, which were detected with Molony virus-induced lymphoma (YAC-1) and radiation-induced leukemia (RL male 1) cells as targets. The same antiserum abolished T cell functions, e.g. carrier-specific helper function and the responsiveness to concanavalin A, but not B cell functions, e.g. immunological memory for the secondary antibody response and the responsiveness to lipopolysaccharide. After absorption of the anti-BAT with thymocytes, the ability to kill T cells was completely abrogated, leaving the activity to kill NK cells intact. No other heterologous and isologous antisera, i.e. rabbit anti-mouse thymocyte antiserum, goat antiserum against antigens shared by thymus and B cells, anti-Thy-1.2 and anti-Ia antisera, could eliminate NK function regardless of their definite reactivity against T or B cells. The results indicate that the absorbed anti-BAT can distinguish NK cells from other known subsets of T and B cells.
抗小鼠脑组织兔抗血清（抗脑相关T细胞抗原，抗-BAT）能够杀死CBA/J、BALB/c、C 57 Bl/6J、C 3 H/He和裸鼠的脾自然杀伤（NK）细胞，这些细胞以莫洛尼病毒诱导的淋巴瘤（YAC-1）细胞和辐射诱导的白血病（RL male 1）细胞作为靶细胞进行检测。相同的抗血清消除了T细胞功能，如载体特异性辅助功能和对刀豆蛋白A的反应性，但未消除B细胞功能，如二次抗体反应的免疫记忆和对脂多糖的反应性。用胸腺细胞吸收抗-BAT后，杀伤T细胞的能力完全丧失，而杀伤NK细胞的活性保持完整。没有其他异种和同种抗血清，即兔抗小鼠胸腺细胞抗血清、抗胸腺和B细胞共有的抗原的山羊抗血清、抗-Thy-1.2和抗-Ia抗血清，能够消除NK功能，无论它们对T细胞或B细胞具有确定的反应性。结果表明，吸收后的抗-BAT能够将NK细胞与T细胞和B细胞的其他已知亚群区分开来。
