钙对鲍曼不动杆菌与人呼吸道上皮细胞相互作用的影响。

Effect of calcium on the interaction of Acinetobacter baumannii with human respiratory epithelial cells.

机构信息

Hangzhou First People's Hospital, Zhejiang Chinese Medical University, Hangzhou, China.

Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, China.

出版信息

BMC Microbiol. 2019 Nov 27;19(1):264. doi: 10.1186/s12866-019-1643-z.

BACKGROUND

Investigating the factors that influence Acinetobacter baumannii(Ab) adhesion/invasion of host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effect of calcium on host-bacterial interaction has yet to be clarified. Here, the dynamic process of the interaction between Ab and human respiratory epithelial cells and the effect of calcium on host-bacterial interaction were explored using microscopic imaging, quantitative PCR and real time cellular analysis (RTCA).

RESULTS

The concentration of calcium, multiplicity of infection and co-culture time were all demonstrated to have effects on host-bacterial interaction. A unique "double peak" phenomenon changed to a sharp "single peak" phenomenon during the process of Ab infection under the effect of calcium was observed in the time-dependent cell response profiles. Moreover, calcium can increase Ab adhesion/invasion of epithelial cells by regulating the expression of Ab-related genes (ompA, bfmRS, abaI).

CONCLUSIONS

Effective control of calcium concentrations can provide new approaches for the prevention and treatment of multi-drug resistant Ab.

背景

研究影响鲍曼不动杆菌（Ab）黏附/侵袭宿主细胞的因素对于了解其致病性很重要。已经表明，金属阳离子在调节生物膜形成和增加 Ab 毒力方面发挥着重要作用；然而，钙对宿主-细菌相互作用的影响尚不清楚。在这里，使用显微镜成像、定量 PCR 和实时细胞分析 (RTCA) 探索了 Ab 与人类呼吸道上皮细胞之间的相互作用的动态过程以及钙对宿主-细菌相互作用的影响。