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NLR 免疫受体中的 N 端基序在亲缘关系较远的植物物种中具有功能保守性。

An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species.

机构信息

The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom.

Department of Life Sciences, Imperial College London, London, United Kingdom.

出版信息

Elife. 2019 Nov 27;8:e49956. doi: 10.7554/eLife.49956.

DOI:10.7554/eLife.49956
PMID:31774397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6944444/
Abstract

The molecular codes underpinning the functions of plant NLR immune receptors are poorly understood. We used in vitro Mu transposition to generate a random truncation library and identify the minimal functional region of NLRs. We applied this method to NRC4-a helper NLR that functions with multiple sensor NLRs within a Solanaceae receptor network. This revealed that the NRC4 N-terminal 29 amino acids are sufficient to induce hypersensitive cell death. This region is defined by the consensus MADAxVSFxVxKLxxLLxxEx (MADA motif) that is conserved at the N-termini of NRC family proteins and ~20% of coiled-coil (CC)-type plant NLRs. The MADA motif matches the N-terminal α1 helix of Arabidopsis NLR protein ZAR1, which undergoes a conformational switch during resistosome activation. Immunoassays revealed that the MADA motif is functionally conserved across NLRs from distantly related plant species. NRC-dependent sensor NLRs lack MADA sequences indicating that this motif has degenerated in sensor NLRs over evolutionary time.

摘要

植物 NLR 免疫受体功能的分子密码知之甚少。我们使用体外 Mu 转座生成随机截断文库，并鉴定 NLR 的最小功能区域。我们将这种方法应用于 NRC4——一种与茄科受体网络中的多个传感器 NLR 一起发挥作用的辅助 NLR。这表明 NRC4 的 N 端 29 个氨基酸足以诱导过敏细胞死亡。该区域由保守的 NRC 家族蛋白和~20%的卷曲螺旋 (CC)-型植物 NLR 的 N 末端的共有 MADAxVSFxVxKLxxLLxxEx (MADA 基序) 定义。MADA 基序与拟南芥 NLR 蛋白 ZAR1 的 N 端α1 螺旋匹配，该螺旋在抵抗体激活过程中发生构象转变。免疫测定显示，MADA 基序在来自不同植物物种的 NLR 中具有功能保守性。依赖 NRC 的传感器 NLR 缺乏 MADA 序列，表明该基序在进化过程中已经在传感器 NLR 中退化。

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