Opt Lett. 2019 Dec 1;44(23):5671-5674. doi: 10.1364/OL.44.005671.
Functional retinal imaging, especially of neuronal activity non-invasively in humans, is of tremendous interest. Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT), we show simultaneous imaging of the activation in the photoreceptor and ganglion cell layer/inner plexiform layer (GCL/IPL). The signals from the GCL/IPL are 10-fold smaller than those from the PRC and were detectable only using algorithms for suppression of motion artifacts and pulsatile blood flow in the retinal vessels. FF-SS-OCT with improved phase evaluation algorithms, therefore, allowed us to map functional connections between PRC and GCL/IPL, confirming previous ex vivo results. The demonstrated functional imaging of retinal neuronal layers can be a valuable tool in diagnostics and basic research.
功能视网膜成像，特别是在人类中对神经元活性进行非侵入性成像，具有巨大的兴趣。虽然已经可以在人类中检测到光感受器细胞 (PRC) 的激活，但迄今为止，对其他视网膜神经元功能的成像几乎是不可能的。在这里，我们使用相敏全视野扫频光学相干断层扫描 (FF-SS-OCT)，同时显示光感受器和神经节细胞层/内丛状层 (GCL/IPL) 的激活。来自 GCL/IPL 的信号比 PRC 的信号小 10 倍，并且仅使用用于抑制视网膜血管中运动伪影和脉动血流的算法才能检测到。因此，具有改进的相位评估算法的 FF-SS-OCT 使我们能够绘制 PRC 和 GCL/IPL 之间的功能连接，证实了以前的离体结果。所展示的视网膜神经元层的功能成像可以成为诊断和基础研究的有价值的工具。
