[14C]丁醇分布：一种测量脊髓血流的新方法。

[14C]butanol distribution: a new method for measurement of spinal cord blood flow.

作者信息

Sakamoto T, Shimazaki S, Monafo W W

机构信息

Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.

出版信息

Am J Physiol. 1988 Oct;255(4 Pt 2):H953-9. doi: 10.1152/ajpheart.1988.255.4.H953.

DOI:10.1152/ajpheart.1988.255.4.H953

PMID:3177685

Abstract

[14C]butanol distribution was used to quantitate regional blood flow (SCBF) in the spinal cord (levels T3-5, T7-9, L1-2, L3-S) and in the sciatic nerves (NBF) of control pentobarbital sodium-anesthetized rats (group A), after 1 h of hemorrhagic hypotension (group B), after 15 min of stimulation of one sciatic nerve (group C-1), and after stimulation of one sciatic nerve plus hemorrhage, which maintained mean arterial pressure (MAP) at control (130 mmHg). Group A SCBF ranged from 52.3 +/- 3.5 (L3-S) to 67.4 +/- 2.7 (L1-2) ml.min-1.100 g-1. NBF was 8.0 +/- 0.9 ml.min-1.100 g-1. Group B SCBF was unchanged. NBF fell to 4.0 +/- 0.4 ml.min-1.100 g-1. Group C-1 SCBF was markedly elevated (range 122 +/- 23.1 to 150.1 +/- 18.7 ml.min-1.100 g-1). NBF was 33.5 +/- 4.1 ml.min-1.100 g-1 (stimulated side) and 14.7 +/- 1.4 ml.min-1.100 g-1 (nonstimulated). MAP was elevated (163 +/- 6 mmHg). In group C-2 (MAP was 130 +/- 4 mmHg), SCBF was still elevated at T3-5, L3-S, and marginally elevated at L1-2. NBF was 22.6 +/- 4.7 ml.min-1.100 g-1 (stimulated) but unchanged contralaterally. [14C]butanol distribution provides a sensitive reproducible measure of SCBF and NBF. Autoregulation of SCBF (but not of NBF) occurred in the range 60-160 mmHg MAP. Spinal cord stimulation via the sciatic nerve increased SCBF two- to threefold, but when hypertension was avoided by blood withdrawal, a modest (38%) increase in SCBF still occurred.

摘要

采用[¹⁴C]丁醇分布法对戊巴比妥钠麻醉的对照大鼠（A组）、出血性低血压1小时后的大鼠（B组）、刺激一侧坐骨神经15分钟后的大鼠（C - 1组）以及刺激一侧坐骨神经加出血且平均动脉压（MAP）维持在对照水平（130 mmHg）的大鼠（C - 2组）的脊髓（T3 - 5、T7 - 9、L1 - 2、L3 - S节段）和坐骨神经（神经血流量，NBF）的局部血流量（脊髓局部血流量，SCBF）进行定量分析。A组的SCBF范围为52.3±3.5（L3 - S节段）至67.4±2.7（L1 - 2节段）ml·min⁻¹·100 g⁻¹。NBF为8.0±0.9 ml·min⁻¹·100 g⁻¹。B组的SCBF未发生变化。NBF降至4.0±0.4 ml·min⁻¹·100 g⁻¹。C - 1组的SCBF显著升高（范围为122±23.1至150.1±18.7 ml·min⁻¹·100 g⁻¹）。NBF在刺激侧为33.5±4.1 ml·min⁻¹·100 g⁻¹，在未刺激侧为14.7±1.4 ml·min⁻¹·100 g⁻¹。MAP升高（163±6 mmHg）。在C - 2组（MAP为130±4 mmHg）中，T3 - 5、L3 - S节段的SCBF仍升高，L1 - 2节段略有升高。NBF在刺激侧为22.6±4.7 ml·min⁻¹·100 g⁻¹，对侧未发生变化。[¹⁴C]丁醇分布为SCBF和NBF提供了一种灵敏且可重复的测量方法。在MAP为60 - 160 mmHg范围内，SCBF存在自身调节（而NBF不存在）。通过坐骨神经刺激脊髓可使SCBF增加2至3倍，但当通过放血避免高血压时，SCBF仍有适度（38%）的增加。