Zou Hanxue, Li Hongxia
Department of Obstetrics and Gynecology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, P.R. China.
Exp Ther Med. 2019 Dec;18(6):4510-4516. doi: 10.3892/etm.2019.8066. Epub 2019 Sep 30.
Drug resistance severely limits the effectiveness of chemotherapeutic treatment in ovarian cancer. The present study aimed to investigate the role of long non-coding RNA LINC00152 (LINC00152) in the cisplatin resistance of ovarian cancer. The expression level of LINC00152 was significantly increased in the ovarian cancer CoC1 and CoC1/DDP cell lines compared with the normal ovarian IOSE-80 cell line. To further investigate the function of LINC00152, small interfering RNAs (siRNAs) targeting LINC00152 were transfected into COC1 and COC1/DDP cells, which were subsequently treated with varying concentrations of cisplatin. The results revealed that LINC00152 silencing increased the apoptotic rates and enhanced the chemosensitivity of CoC1 and CoC1/DDP cells to cisplatin. Furthermore, downregulation of LINC00152 significantly decreased Bcl-2, and increased Bax and cleaved caspase-3 expression levels. Additionally, LINC00152 silencing decreased the expression of multidrug resistance-associated gene 1 (MDR1), multidrug resistance-associated protein 1 (MRP1) and glutathione S-transferase π (GSTπ). Collectively, the data demonstrated that LINC00152 knockdown increased the chemosensitivity of epithelial ovarian cancer cells to cisplatin by increasing apoptosis and decreasing the expression levels of MDR1, MRP1 and GSTπ.
耐药性严重限制了化疗在卵巢癌治疗中的有效性。本研究旨在探讨长链非编码RNA LINC00152（LINC00152）在卵巢癌顺铂耐药中的作用。与正常卵巢IOSE - 80细胞系相比，LINC00152在卵巢癌CoC1和CoC1/DDP细胞系中的表达水平显著升高。为进一步研究LINC00152的功能，将靶向LINC00152的小干扰RNA（siRNA）转染到COC1和COC1/DDP细胞中，随后用不同浓度的顺铂处理这些细胞。结果显示，LINC00152沉默增加了CoC1和CoC1/DDP细胞的凋亡率，并增强了它们对顺铂的化疗敏感性。此外，LINC00152的下调显著降低了Bcl - 2的表达，并增加了Bax和裂解的caspase - 3的表达水平。另外，LINC00152沉默降低了多药耐药相关基因1（MDR1）、多药耐药相关蛋白1（MRP1）和谷胱甘肽S - 转移酶π（GSTπ）的表达。总的来说，数据表明LINC00152的敲低通过增加凋亡以及降低MDR1、MRP1和GSTπ的表达水平，提高了上皮性卵巢癌细胞对顺铂的化疗敏感性。
