KIAA1211在人类非小细胞肺癌中发挥致癌作用。
KIAA1211 plays an oncogenic role in human non-small cell lung cancer.
作者信息
Liu Zhengcheng, Cao Hui, Shi Ye, Yang Rusong
机构信息
Department of Thoracic Surgery, Nanjing Chest Hospital, Nanjing, Jiangsu, 210000, China.
出版信息
J Cancer. 2019 Oct 22;10(26):6747-6753. doi: 10.7150/jca.35951. eCollection 2019.
One of the main causes of cancer disease and death worldwide is lung cancer. Our study focused on the function of KIAA1211 in non-small cell lung cancer (NSCLC). According to the data about NSCLC patients that from the Cancer Genome Atlas (TCGA), we found that KIAA1211 in NSCLC (P=5.06E-06) was significantly higher than the adjacent normal. Lentivirus-mediated short hairpin RNA (shRNA) was used to knockdown BATF expression in the human A549 NSCLC cell line and assessed by RT-qPCR and Western blot. Cell proliferation was evaluated by MTT assay and Celigo imaging cytometry. Cell apoptosis were detected by Annexin V staining. The test results showed that KIAA1211-shRNA A549 and SPC-A-1 cells can inhibit cell proliferation, and the apoptosis rate of KIAA1211-shRNA group was significantly higher than that of the control group. Knockdown of KIAA1211 inhibited NSCLC progression in xenograft tumor model. In conclusion, KIAA1211 could regulate NSCLC cells proliferation and apoptosis and . KIAA1211 may serve as a potent target for the treatment of NSCLC.
全球范围内，癌症疾病和死亡的主要原因之一是肺癌。我们的研究聚焦于KIAA1211在非小细胞肺癌（NSCLC）中的功能。根据来自癌症基因组图谱（TCGA）的非小细胞肺癌患者数据，我们发现非小细胞肺癌中的KIAA1211（P = 5.06E - 06）显著高于相邻正常组织。利用慢病毒介导的短发夹RNA（shRNA）敲低人A549非小细胞肺癌细胞系中的BATF表达，并通过RT - qPCR和蛋白质免疫印迹法进行评估。通过MTT法和Celigo成像细胞术评估细胞增殖。通过膜联蛋白V染色检测细胞凋亡。测试结果表明，KIAA1211 - shRNA A549和SPC - A - 1细胞可抑制细胞增殖，且KIAA1211 - shRNA组的凋亡率显著高于对照组。在异种移植肿瘤模型中，敲低KIAA1211可抑制非小细胞肺癌进展。总之，KIAA1211可调节非小细胞肺癌细胞的增殖和凋亡，并且KIAA1211可能成为治疗非小细胞肺癌的有效靶点。
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