噬菌体疗法作为控制鼠伤寒沙门氏菌血清型感染的方法。
Phage Therapy as an Approach to Control Salmonella enterica serotype Enteritidis Infection in Mice.
机构信息
Division of Food Microbiology, Department of Pathobiology, School of Public Health (TUMS), Tehran, Iran.
Food Microbiology Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
出版信息
Rev Soc Bras Med Trop. 2019 Nov 14;52:e20190290. doi: 10.1590/0037-8682-0290-2019. eCollection 2019.
INTRODUCTION
Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages.
METHODS
S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo.
RESULTS
The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages.
CONCLUSIONS
The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.
简介
肠炎沙门氏菌（S. Enteritidis）是食源性人类疾病的病原体。鉴于沙门氏菌肠炎的抗生素耐药性普遍存在，以及未来几年抗生素疗效缺乏，有必要用其他药物替代。其中最有用的药物之一是噬菌体。
方法
采用多重聚合酶链反应检测法鉴定肠炎沙门氏菌。从医院废水样本中分离出有效的噬菌体。评估噬菌体在体外和体内的效果。
结果
噬菌体 SE20 属于肌尾噬菌体科，基因组大小为 40kb。噬菌体 SE20 在不同 pH 值范围下的评估表明，它对 pH<3 和 pH>12 敏感。动物模型表明，感染肠炎沙门氏菌的小鼠会出现肝脾肿大，但在接受噬菌体后不会出现胃肠道并发症。
结论
本研究结果表明，噬菌体 SE20 是控制肠炎沙门氏菌引起的沙门氏菌病的有前途的候选药物。
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