Bao Hongduo, Zhou Yan, Shahin Khashayar, Zhang Hui, Cao Fuliang, Pang Maoda, Zhang Xuhui, Zhu Shujiao, Olaniran Ademola, Schmidt Stefan, Wang Ran
Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China; College of Forestry, Nanjing Forestry University, Nanjing, 210037, China; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban, 4000, South Africa; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Pietermaritzburg Campus, Private Bag X01, Pietermaritzburg, 3201, South Africa.
Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Microbiol Res. 2020 Aug;237:126471. doi: 10.1016/j.micres.2020.126471. Epub 2020 Mar 19.
S. Enteritidis continues to be the most common pathogen of farm animals and a major public health burden worldwide. Using bacteriophages is a potential alternative to antibiotics against S. Enteritidis infection. In this study, the genome analysis of the lytic phage vB_SenM-PA13076 (PA13076) infecting S. Enteritidis revealed a linear, double-stranded DNA genome, which comprised of 52,474 bp and contained 69 ORFs. It belongs to the order Caudovirales; family Myoviridae, genus unclassified. The genes coded for DNA packaging, phage structural proteins, lysis components, DNA recombination, regulation, modification, and replication. No bacterial virulence or drug-resistance genes were detected. The phage PA13076 protected mice from a lethal dose of S. Enteritidis 13076 (5 × 10 CFU) by reducing the concentration of bacterial cells in blood, intestine, liver, spleen, and kidney. The phage PA13076 achieved at least 2.5 log reductions of S. Enteritidis cells in infected mice within 24 h (P < 0.05) when compared to the organs of control mice. The data also indicated that phage PA13076 could rapidly enter the blood and four organs of infected mice, remaining therein at concentrations of>10 PFU/g for at least 72 h. These results show that phage PA13076 has definite potential as an antibacterial therapeutic agent for attenuating S. Enteritidis infections.
肠炎沙门氏菌仍然是农场动物中最常见的病原体，也是全球主要的公共卫生负担。使用噬菌体是对抗肠炎沙门氏菌感染的一种潜在抗生素替代方法。在本研究中，对感染肠炎沙门氏菌的裂解性噬菌体vB_SenM-PA13076（PA13076）进行的基因组分析揭示了一个线性双链DNA基因组，其由52474个碱基对组成，包含69个开放阅读框。它属于有尾噬菌体目；肌尾噬菌体科，未分类属。这些基因编码DNA包装、噬菌体结构蛋白、裂解成分、DNA重组、调控、修饰和复制。未检测到细菌毒力或耐药基因。噬菌体PA13076通过降低血液、肠道、肝脏、脾脏和肾脏中的细菌细胞浓度，保护小鼠免受致死剂量的肠炎沙门氏菌13076（5×10⁸CFU）感染。与对照小鼠的器官相比，噬菌体PA13076在24小时内使感染小鼠体内的肠炎沙门氏菌细胞至少减少2.5个对数（P<0.05）。数据还表明，噬菌体PA13076可迅速进入感染小鼠的血液和四个器官，并在其中以>10⁶PFU/g的浓度至少保持72小时。这些结果表明，噬菌体PA13076作为一种减轻肠炎沙门氏菌感染的抗菌治疗剂具有一定潜力。
