Knuth U A, Kühne J, Bals-Pratsch M, Nieschlag E
Max Planck Clinical Research Unit for Reproductive Medicine University, Münster, Federal Republic of Germany.
Andrologia. 1988 May-Jun;20(3):243-8.
In an effort to evaluate the intra-individual fluctuations of sperm motion parameters 12 healthy young men with normal semen parameters were recruited. Weekly semen analysis was performed for 13 weeks. Conventional semen parameters such as sperm concentration, percentage of motile and normally formed sperm were determined according to WHO guidelines, while sperm velocity, linearity, lateral head displacement and beat frequency were evaluated by computerized image analysis. Mean and standard deviations (SD) of all parameters were computed for each individual. Coefficients of variation were expressed as the ratio of SD/mean. On average the following coefficients of variation were found: total number of sperm 58 (+/- 5%); concentration of sperm 47 (+/- 4%); lateral head displacement 27 (+/- 4%); volume 24 (+/- 3%); velocity 19 (+/- 2%); linearity 17 (+/- 2%); percentage of motile sperm 12 (+/- 2%); beat frequency: 12 (+/- 2%) and percentage of normally formed sperm 9 (+/- 1%). The low variation of sperm motion parameters and the ease of their determination suggests that computerized motion analysis of sperm may be a valuable research tool to detect subtle effects e.g. of drugs and environmental toxins on reproductive functions in men.
为了评估精子运动参数的个体内波动情况，招募了12名精液参数正常的健康年轻男性。在13周内每周进行一次精液分析。根据世界卫生组织指南测定常规精液参数，如精子浓度、活动精子百分比和正常形态精子百分比，同时通过计算机图像分析评估精子速度、直线性、头部横向位移和摆动频率。计算每个个体所有参数的平均值和标准差（SD）。变异系数表示为SD/平均值的比值。平均发现以下变异系数：精子总数58（±5%）；精子浓度47（±4%）；头部横向位移27（±4%）；体积24（±3%）；速度19（±2%）；直线性17（±2%）；活动精子百分比12（±2%）；摆动频率12（±2%）；正常形态精子百分比9（±1%）。精子运动参数的低变异性及其测定的简便性表明，精子的计算机化运动分析可能是检测药物和环境毒素等对男性生殖功能的细微影响的有价值的研究工具。
