Kumar Davendra, Joshi Anil, Naqvi S M K, Kumar S, Mishra A K, Maurya V P, Arora A L, Mittal J P, Singh V K
Division of Animal Physiology and Biochemistry, Central Sheep and Wool Research Institute, Avikanagar, Via Jaipur, Rajasthan 304501, India.
Anim Reprod Sci. 2007 Jul;100(1-2):51-60. doi: 10.1016/j.anireprosci.2006.07.004. Epub 2006 Sep 5.
The arid and semi-arid tropical climates of India are endowed with vast diversity of non-prolific sheep breeds. The GarolexMalpura sheep has been evolved in a semi-arid tropical environment by introgression of FecB gene via artificial insemination of Malpura ewes using diluted semen of prolific microsheep Garole and subsequently multiplied by inter se mating among GarolexMalpura halfbreds. The aim of the present study was to identify FecB mutation in sexually mature GarolexMalpura rams by forced RFLP-PCR of BMPR-1B gene and evaluate: (i) semen production and sperm motion characteristics of GM rams and (ii) influence of age and FecB genotype on their semen attributes. Semen was collected during autumn season from 12 donor rams by artificial vagina on 8 occasions at weekly interval. The overall means of traits which did not differed significantly with age or FecB genotyping were volume (0.72 ml), mass motility (4.44), sperm concentration (2721.56 x 10(6)ml(-1)), curvilinear velocity (134.51 microm/s), motility (81.3%), amplitude of lateral head displacement (6.24 microm), beat frequency (44.43 Hz), sperm head elongation (48.9%) and sperm head area (10.01 microm(2)). The FecB genotyping had a significant effect (P<0.05) on percent linearity and rapid motile sperms, which did not vary significantly with age. Although sperm concentration was higher in FecB(BB) and FecB(B+), compared to FecB(++) genotypes but the effect was non-significant. The age and FecB genotyping had significant effects (P<0.05) on straightness, average path velocity, straight-line velocity and percentage of medium or slow motile sperms. It is concluded that GarolexMalpura rams with introgressed FecB gene are capable of producing good quality semen in a semi-arid tropical climate.
印度的干旱和半干旱热带气候孕育了种类繁多的低产绵羊品种。加罗尔×马尔普拉绵羊是在半干旱热带环境中，通过使用多产微型绵羊加罗尔的稀释精液对马尔普拉母羊进行人工授精，将FecB基因渗入其中，随后通过加罗尔×马尔普拉杂种羊之间的相互交配繁殖而来。本研究的目的是通过对BMPR - 1B基因进行强制限制性片段长度多态性聚合酶链反应（RFLP - PCR），鉴定性成熟的加罗尔×马尔普拉公羊中的FecB突变，并评估：（i）GM公羊的精液产量和精子运动特征，以及（ii）年龄和FecB基因型对其精液特性的影响。在秋季，通过人工阴道，每周一次，在8个不同时间从12只供体公羊采集精液。与年龄或FecB基因分型无显著差异的性状总体均值分别为：体积（0.72毫升）、总体活力（4.44）、精子浓度（2721.56×10⁶毫升⁻¹）、曲线速度（134.51微米/秒）、活力（81.3%）、头部侧向位移幅度（6.24微米）、拍频（44.43赫兹）、精子头部伸长率（48.9%）和精子头部面积（10.01微米²）。FecB基因分型对线性度百分比和快速运动精子有显著影响（P<0.05），且该影响不随年龄显著变化。尽管FecB（BB）和FecB（B +）基因型的精子浓度高于FecB（++）基因型，但差异不显著。年龄和FecB基因分型对直线度、平均路径速度、直线速度以及中等或缓慢运动精子的百分比有显著影响（P<0.05）。研究得出结论，导入FecB基因的加罗尔×马尔普拉公羊能够在半干旱热带气候下生产出优质精液。
