Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Sarriena s/n, 48940 Leioa, Biscay, Basque Country, Spain.
Molecules. 2019 Nov 26;24(23):4320. doi: 10.3390/molecules24234320.
Dried blood spot (DBS) has lately experienced an increase in its use in bioanalysis due to its several advantages compared with traditional blood sampling methods. Nevertheless, the use of DBS with quantitative purposes is hindered by the heterogeneous distribution of some compounds in the supporting matrix and the dependence of the response on different factors, such as the hematocrit, blood volume, and sampling position. In this study the effect of those factors in the analytical response was investigated by ultra high performance liquid chromatography coupled to fluorescence detection, using amiloride and propranolol as model compounds. The results showed a heterogeneous and drug-dependent distribution of the compounds in the blood spot. While amiloride concentration was higher in the center, propranolol concentration was higher in the periphery of the spot. Besides, the influence of the hematocrit on the quantitative results was observed. MALDI mass spectrometry imaging (MALDI-IMS) has allowed study of the distribution of the two cardiovascular drugs when they were placed in the DBS card using water:methanol solutions, demonstrating that they followed a similar distribution pattern as in blood. This work has showed the potentiality of the MALDI-IMS technique to predict the distribution of the drugs in the DBS card.
干血斑（DBS）由于与传统采血方法相比具有许多优势，最近在生物分析中得到了越来越多的应用。然而，由于某些化合物在支持基质中的不均匀分布以及响应对不同因素（如红细胞压积、血量和采样位置）的依赖性，DBS 用于定量目的受到阻碍。在这项研究中，使用阿米洛利和普萘洛尔作为模型化合物，通过超高效液相色谱法结合荧光检测法研究了这些因素对分析响应的影响。结果表明，化合物在血斑中的分布不均匀且具有药物依赖性。阿米洛利的浓度在中央较高，而普萘洛尔的浓度在斑点的外围较高。此外，还观察到红细胞压积对定量结果的影响。基质辅助激光解吸电离质谱成像（MALDI-IMS）允许使用水：甲醇溶液研究两种心血管药物在 DBS 卡上的分布，证明它们的分布模式与血液中的相似。这项工作表明 MALDI-IMS 技术有潜力预测药物在 DBS 卡中的分布。
