利用转基因细菌修复混凝土裂缝。
Use of Genetically Modified Bacteria to Repair Cracks in Concrete.
作者信息
Zhang Zhigang, Weng Yiwei, Ding Yuanzhao, Qian Shunzhi
机构信息
Key Laboratory of New Technology for Construction of Cities in Mountain Area, Chongqing University, Ministry of Education, Chongqing 400045, China.
School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 637551, Singapore.
出版信息
Materials (Basel). 2019 Nov 26;12(23):3912. doi: 10.3390/ma12233912.
In this paper, we studied the crack-repair by spraying bacteria-based liquid around the cracks in concrete. To enhance the repair efficiency and speed up the repair process, the transposon mutagenesis method was employed to modify the genes of and create a mutant bacterial strain with higher efficiency of calcium carbonate productivity by catalyzing the combination of carbonate and calcium ion. The efficiency of crack-repairing in concrete by spraying two kinds of bacterial liquid was evaluated via image analysis, X-ray computed tomography (X-CT) scanning technology and the sorptivity test. The results show that the crack-repair efficiency was enhanced very evidently by spraying genetically modified bacterial-liquid as no microbiologically induced calcite precipitation (MICP) was found within the cracks for concrete samples sprayed using wild type bacterial-liquid. In addition, the crack-repair process was also shortened significantly in the case of genetically modified bacteria.
在本文中，我们研究了通过在混凝土裂缝周围喷洒基于细菌的液体来修复裂缝的方法。为提高修复效率并加快修复过程，采用转座子诱变方法对[具体细菌名称未给出]的基因进行修饰，从而创建了一种突变菌株，该菌株通过催化碳酸盐与钙离子的结合，具有更高的碳酸钙生成效率。通过图像分析、X射线计算机断层扫描（X-CT）技术和吸水率试验，评估了喷洒两种细菌液体对混凝土裂缝的修复效果。结果表明，喷洒转基因细菌液体可显著提高裂缝修复效率，因为使用野生型细菌液体喷洒的混凝土样品裂缝内未发现微生物诱导碳酸钙沉淀（MICP）。此外，使用转基因细菌时，裂缝修复过程也显著缩短。
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