构建和表征用于人类感染噬菌体治疗的噬菌体文库。
Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections.
作者信息
Gibson Shelley B, Green Sabrina I, Liu Carmen Gu, Salazar Keiko C, Clark Justin R, Terwilliger Austen L, Kaplan Heidi B, Maresso Anthony W, Trautner Barbara W, Ramig Robert F
机构信息
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States.
Department of Microbiology and Molecular Genetics, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, United States.
出版信息
Front Microbiol. 2019 Nov 12;10:2537. doi: 10.3389/fmicb.2019.02537. eCollection 2019.
Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: , , and . The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and virulence on a number of contemporary clinical isolates are presented. This information is required before phages can be added to a phage library for potential human use or sharing between laboratories for use in compassionate use protocols in humans under eIND (emergency investigational new drug). Clinical scenarios in which these phages can potentially be used are discussed. The phages presented here are currently being characterized in animal models and are available for eINDs.
噬菌体疗法需要特征明确的噬菌体文库。在此，我们描述了针对三种目标物种（此处原文缺失具体物种名称）生成噬菌体文库的过程。介绍了在分离宿主上的基本噬菌体特征、序列分析、生长特性以及对一些当代临床分离株的宿主范围和毒力。在将噬菌体添加到可能供人类使用的噬菌体文库或在实验室之间共享以用于根据紧急研究性新药（eIND）进行的人类同情用药方案之前，需要这些信息。讨论了这些噬菌体可能适用的临床场景。此处介绍的噬菌体目前正在动物模型中进行特征鉴定，并且可用于紧急研究性新药申请。
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