Abdo Ahmad Tasnime A, El Houjeiry Samar A, Abou Fayad Antoine, Kanj Souha S, Matar Ghassan M, Saba Esber S
Department of Experimental Pathology, Immunology, and Microbiology, Faculty of Medicine, Center for Infectious Diseases Research, American University of Beirut, Beirut 1107 2020, Lebanon.
Division of Infectious Diseases, Department of Internal Medicine, Center for Infectious Diseases Research, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.
Antibiotics (Basel). 2025 Apr 30;14(5):458. doi: 10.3390/antibiotics14050458.
BACKGROUND/OBJECTIVES: (), a prevalent Gram-negative bacterium, is a frequent cause of illness. The extensive use of antibiotics has led to the emergence of resistant strains, complicating antimicrobial therapy and emphasizing the need for natural alternatives such as phages.
In this study, a novel phage, AUBRB02, was isolated from sewage and characterized through whole-genome sequencing, host range assays, and biofilm elimination assays. The phage's stability and infectivity were assessed under various pH and temperature conditions, and different strains.
Phage AUBRB02 has an incubation period of 45 min, a lysis period of 10 min, and a burst size of 30 phages/infected cell. It is stable across pH 5.0-9.0 and temperatures from 4 °C to 60 °C. Treatment with AUBRB02 significantly reduced post-formation biofilms, as indicated by lower OD values compared with the positive control. The whole genome sequencing revealed a genome size of 166,871 base pairs with a G + C (Guanine and Cytosine content) content of 35.47%. AUBRB02 belongs to the genus, sharing 93% intergenomic similarity with its closest RefSeq relative, and encodes 262 coding sequences, including 10 tRNAs.
AUBRB02 demonstrates high infectivity and stability under diverse conditions. Its genomic features and similarity to related phages highlight its potential for phage therapy, offering promising prospects for the targeted treatment of infections.
背景/目的：（某种细菌名称未给出）是一种常见的革兰氏阴性菌，是引发疾病的常见原因。抗生素的广泛使用导致了耐药菌株的出现，使抗菌治疗变得复杂，凸显了对噬菌体等天然替代品的需求。
在本研究中，从污水中分离出一种新型噬菌体AUBRB02，并通过全基因组测序、宿主范围测定和生物膜消除测定对其进行表征。在各种pH和温度条件下以及不同菌株中评估了噬菌体的稳定性和感染性。
噬菌体AUBRB02的潜伏期为45分钟，裂解期为10分钟，爆发量为每个感染细胞产生30个噬菌体。它在pH 5.0至9.0以及4℃至60℃的温度范围内稳定。与阳性对照相比，AUBRB02处理显著降低了生物膜形成后的生物膜量，表现为较低的OD值。全基因组测序显示基因组大小为166,871个碱基对，鸟嘌呤和胞嘧啶含量（G + C含量）为35.47%。AUBRB02属于（某个属名称未给出）属，与其最接近的RefSeq亲属的基因组间相似性为93%，并编码262个编码序列，包括10个tRNA。
AUBRB02在不同条件下表现出高感染性和稳定性。其基因组特征以及与相关噬菌体的相似性突出了其在噬菌体治疗中的潜力，为针对性治疗（某种细菌感染名称未给出）感染提供了有前景的前景。
