School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
The Oil Crops Research Institute (OCRI) of the Chinese Academy of Agricultural Sciences (CAAS), Wuhan, China.
Plant Physiol Biochem. 2020 Jan;146:294-302. doi: 10.1016/j.plaphy.2019.11.026. Epub 2019 Nov 19.
Brassica napus (rapeseed) serves as a main source of edible oil, and the oil's quality is mainly determined by the relative proportions of fatty acids. A high oleic acid concentration in B. napus oil increases its shelf life and oxidative stability. Therefore, attaining a high oleic acid concentration is necessary to enhance the nutritional quality of rapeseed oil. Here, an association study of candidate genes was conducted using a population of 324 genetically diverse rapeseed accessions, and several loci related to oleic acid content were identified. Furthermore, these loci were functionally characterized in Saccharomyces cerevisiae to assess their functions, and the promising candidate loci were validated using single nucleotide polymorphic markers in an independent inbred population. The results increased our understanding of fatty acid metabolism in B. napus. Moreover, these findings may assist in marker-based breeding efforts to improve the fatty acid composition and quality of B. napus oil.
油菜（甘蓝型油菜）是食用油的主要来源，其油质主要取决于脂肪酸的相对比例。油菜籽油中高油酸的浓度可以延长其保质期和抗氧化稳定性。因此，提高油菜籽油中油酸的浓度对于提高其营养价值是必要的。在这里，我们使用一个由 324 个遗传多样性的油菜品种组成的群体进行了候选基因的关联研究，鉴定出了与油酸含量相关的几个基因座。此外，我们在酿酒酵母中对这些基因座进行了功能表征，以评估它们的功能，并使用单核苷酸多态性标记在一个独立的自交群体中对有希望的候选基因座进行了验证。这些结果增加了我们对油菜脂肪酸代谢的认识。此外，这些发现可能有助于基于标记的油菜脂肪酸组成和品质改良的育种工作。
