School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
The Oil Crops Research Institute (OCRI) of the Chinese Academy of Agricultural Sciences (CAAS), Wuhan, China.
J Plant Physiol. 2020 May;248:153159. doi: 10.1016/j.jplph.2020.153159. Epub 2020 Mar 23.
Due to the rapid decline in oceanic fish stock, ω-3 fatty acid (C18:3) has attracted serious attention and, hence, the identification of genotypes with high ω-3 content has become the main objective of Brassica napus (rapeseed) breeding. A candidate genes association study permitted us to delineate a genomic region linked to ω-3 content, offering a detailed understanding of the complex genetic mechanism of fatty acid biosynthesis in B. napus. Herein, the candidate genes association study, conducted on 324 genetically diverse rapeseed accessions, detected 114 single nucleotide polymorphisms (SNPs) associated with ω-3 fatty acid. Furthermore, these loci were functionally characterized in Saccharomyces cerevisiae. These associated loci were selected based on their contribution to a high C18:3 ratio, and the selected candidate loci were validated using allele-specific SNPs markers in an inbred population through polymerase chain reaction (PCR). These findings may contribute to improving the fatty acid composition by marker-based breeding and will facilitate the development of rapeseed varieties with high ω-3 content.
由于海洋鱼类资源的迅速减少，ω-3 脂肪酸（C18:3）引起了人们的高度关注，因此，鉴定出 ω-3 含量高的基因型已成为油菜（油菜籽）育种的主要目标。候选基因关联研究使我们能够描绘与 ω-3 含量相关的基因组区域，从而深入了解油菜中脂肪酸生物合成的复杂遗传机制。在此，对 324 种遗传多样性的油菜品种进行了候选基因关联研究，检测到与 ω-3 脂肪酸相关的 114 个单核苷酸多态性（SNP）。此外，还在酿酒酵母中对这些基因座进行了功能表征。这些相关基因座是根据其对高 C18:3 比例的贡献选择的，并且通过聚合酶链反应（PCR）在自交群体中使用等位基因特异性 SNP 标记对所选候选基因座进行了验证。这些发现可能有助于通过基于标记的育种来改善脂肪酸组成，并促进开发高 ω-3 含量的油菜品种。
