Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Agronomy College, Jiangxi Agricultural University, Nanchang, China.
Southern Cross Plant Science, Southern Cross University, Lismore, NSW, Australia.
PLoS One. 2019 Aug 23;14(8):e0221578. doi: 10.1371/journal.pone.0221578. eCollection 2019.
Rapeseed oil (canola, Brassica napus L.) is an important healthy vegetable oil throughout the world, the nutritional and economical value of which largely depends on its seed fatty acid composition. In this study, based on 201,187 SNP markers developed from the SLAF-seq (specific locus amplified fragment sequencing), a genome wide association study of four important fatty acid content traits (erucic acid, oleic acid, linoleic acid and linolenic acid) in a panel of 300 inbred lines of rapeseed in two environments (JXAU and JXRIS) was carried out. A total of 148 SNP loci significantly associated with these traits were detected by MLM model analysis respectively, and 30 SNP loci on A08 and C03 chromosomes were detected in three traits of erucic acid, oleic acid and linoleic acid contents simultaneously. Furthermore, 108 highly favorable alleles for increasing oleic acid and linoleic acid content, also for decreasing erucic acid content simultaneously were observed. By a basic local alignment search tool (BLAST) search with in a distance of 100 Kb around these significantly SNP-trait associations, we identified 20 orthologs of the functional candidate genes related to fatty acid biosynthesis, including the known vital fatty acid biosynthesis genes of BnaA.FAE1 and BnaC. FAE1 on the A08 and C03 chromosomes, and other potential candidate genes involving in the fatty acid biosynthesis pathway, such as the orthologs genes of FAD2, LACS09, KCS17, CER4, TT16 and ACBP5. This study lays a basis for uncovering the genetic variations and the improvement of fatty acid composition in B. napus.
菜籽油（油菜，甘蓝型油菜）是全世界重要的健康蔬菜油，其营养价值和经济价值在很大程度上取决于其种子脂肪酸组成。本研究基于来自 SLAF-seq（特异位点扩增片段测序）的 201187 个 SNP 标记，利用 MLM 模型分析，在两个环境（JXAU 和 JXRIS）下对 300 个油菜自交系群体的四个重要脂肪酸含量性状（芥酸、油酸、亚油酸和亚麻酸）进行了全基因组关联研究。共检测到 148 个与这些性状显著相关的 SNP 位点，在芥酸、油酸和亚油酸含量的三个性状中同时检测到 A08 和 C03 染色体上的 30 个 SNP 位点。此外，还观察到 108 个有利于增加油酸和亚油酸含量、同时降低芥酸含量的有利等位基因。通过在这些显著 SNP-性状关联的 100 Kb 范围内进行基本局部比对搜索工具（BLAST）搜索，我们鉴定了 20 个与脂肪酸生物合成相关的功能候选基因的直系同源物，包括 A08 和 C03 染色体上已知的重要脂肪酸生物合成基因 BnaA.FAE1 和 BnaC.FAE1，以及其他潜在的候选基因，如 FAD2、LACS09、KCS17、CER4、TT16 和 ACBP5 的直系同源基因。这项研究为揭示甘蓝型油菜脂肪酸组成的遗传变异和改良奠定了基础。
