单体和六聚体解旋酶的转位不同机制。
Different mechanisms for translocation by monomeric and hexameric helicases.
机构信息
Department of Biosciences, Rice University, Houston, TX 77030, USA.
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Curr Opin Struct Biol. 2020 Apr;61:25-32. doi: 10.1016/j.sbi.2019.10.003. Epub 2019 Nov 26.
Abstract
Helicases are ATP-dependent motor proteins that translocate along single-stranded or double-stranded nucleic acids to alter base-pairing structures or molecular interactions. Helicases can be divided to monomeric and hexameric types, each with distinct ternary structures, nucleic acid-binding modes, and translocation mechanisms. It is well established that monomeric helicases translocate by the inchworm mechanism. Recent structures of different superfamilies of hexameric helicases reveal that they use a hand-over hand mechanism for translocation. Structures of bacteriophage T7 replisome illustrate how helicase and polymerase cooperatively catalyze DNA unwinding. In this review, we survey structures of monomeric and hexameric helicases and compare different mechanisms for translocation.
摘要
解旋酶是一种依赖于 ATP 的分子马达，能沿着单链或双链核酸移动，改变碱基配对结构或分子相互作用。解旋酶可分为单体和六聚体两种类型，每种类型都具有独特的三元结构、核酸结合模式和易位机制。众所周知，单体解旋酶通过尺蠖运动机制进行易位。最近不同超家族六聚体解旋酶的结构揭示，它们使用交替机制进行易位。噬菌体 T7 复制体的结构说明了解旋酶和聚合酶如何协同催化 DNA 解旋。在这篇综述中，我们调查了单体和六聚体解旋酶的结构，并比较了不同的易位机制。
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