Bouteille B, Darde M L, Pestre-Alexandre M, Dumas M, Catanzano G, Breton J C, Nicolas A, Munoz M
Service de Parasitologie, Faculté de Médecine, Limoges, France.
Ann Trop Med Parasitol. 1988 Apr;82(2):149-58. doi: 10.1080/00034983.1988.11812222.
Sheep that have been experimentally infected with a strain of Trypanosoma brucei brucei reproduce the clinical symptomatology of human African trypanosomiasis. We measured two biochemical parameters which are altered during the infection; total serum and cerebrospinal fluid (CSF) proteins, and the time of appearance and levels of IgM in both serum and CSF. Serum protein levels were considerably raised (up to 90 gl-1), although in CSF the levels were always less than 1 gl-1. The ratio of serum albumin to globulins fell to a value of 0.40 during the course of the disease. Electrophoresis of CSF proteins demonstrated the appearance of polyclonal immunoglobulins. Plasma IgM levels rose to ten times normal values, and after the sixth week IgMs made up 10% of total CSF protein content. Specific antibodies were detected, by both indirect immunofluorescence and an ELISA technique, within two weeks after infection in the blood and after four weeks in the CSF.
经实验感染布氏布氏锥虫菌株的绵羊会重现人类非洲锥虫病的临床症状。我们测量了感染期间发生变化的两个生化参数；血清和脑脊液（CSF）总蛋白，以及血清和脑脊液中IgM的出现时间和水平。血清蛋白水平显著升高（高达90 g/L），尽管脑脊液中的水平始终低于1 g/L。在疾病过程中，血清白蛋白与球蛋白的比例降至0.40。脑脊液蛋白电泳显示多克隆免疫球蛋白的出现。血浆IgM水平升至正常值的十倍，六周后IgM占脑脊液总蛋白含量的10%。通过间接免疫荧光和ELISA技术，在感染后两周内在血液中以及四周后在脑脊液中检测到特异性抗体。
