Anhui Laboratory of Molecule-Based Materials; Key Laboratory of Functional Molecular Solids, Ministry of Education; Anhui Key Laboratory of Chemo-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People's Republic of China. Anhui Meijia New Materials Company Limited, Wuhu, 241000, People's Republic of China.
Methods Appl Fluoresc. 2019 Dec 23;8(1):015005. doi: 10.1088/2050-6120/ab5d8c.
In this paper, a simple and convenient fluorescence method for detection of uric acid (UA) based on Ag-doped carbon quantum dots (Ag-CQDs) is developed. The Ag-CQDs contain Ag species could bond with UA, which promoted the electron and/or energy transfer and produced high quenching extent. Thus, the fluorescence intensity of Ag-CQDs decreased in the presence of UA. Under the optimal condition, the UA sensor exhibited a linear response in the range of 0.005-100 μM with a detection limit of 0.35 nM (S/N = 3). Ag-CQDs demonstrated high sensitivity and rapid response to UA over a wide range of concentration indicating their great potential as a fluorescent probe for chemical sensing. Moreover, this method has successfully applied to detect UA in human urine samples.
本文提出了一种基于掺银碳量子点（Ag-CQDs）的简单、便捷的荧光法检测尿酸（UA）的方法。Ag-CQDs 中含有的 Ag 物种可以与 UA 键合，从而促进电子和/或能量转移，并产生高程度的猝灭。因此，在 UA 的存在下，Ag-CQDs 的荧光强度降低。在最佳条件下，UA 传感器在 0.005-100 μM 的范围内呈现线性响应，检测限为 0.35 nM（S/N = 3）。Ag-CQDs 对 UA 表现出高灵敏度和快速响应，浓度范围广泛，表明它们在化学传感领域具有巨大的应用潜力。此外，该方法已成功应用于检测人尿液样品中的 UA。
